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**Profile [#x16afbc6]
&br;
|Name|BGCOLOR(#FFFFFF):''Hiroshi Yoshikawa''|
|Major|BGCOLOR(#FFFFFF):|
|Hobby|BGCOLOR(#FFFFFF):Travel|
|Belong|BGCOLOR(#FFFFFF):Department of Chemistry, Faculty of Science, Saitama University|
|Contact|BGCOLOR(#FFFFFF):Department of Chemistry, Faculty of Science, Saitama University,&br;Shimo-Ohkubo 255, Sakura-Ku, Saitama City, 338-8570, Japan.&br;''E-mail:''hiroshi''"at"''mail.saitama-u.ac.jp|
|Brief History|BGCOLOR(#FFFFFF):2014 - Present Associate Professor, Department of Chemistry, Saitama University, Japan&br;&br;2011 - 2014 Assistant Professor, Department of Chemistry, Saitama University, Japan&br;&br;2010 - 2011 Research Assistant. Department of Physics, St. Francis Xavier University, Canada&br;&br;2007 - 2011 Research Fellow Awarded by the Alexander von Humboldt Foundation, Physical Chemistry of Biosystems, Institute of Physical Chemistry, University of Heidelberg, Germany&br;&br;2006 - 2007 Postdoctoral Fellow, Division of Electrical, Electronic, and Information Technology, Osaka University, Japan&br;&br;2003 - 2006 Department of Applied Physics, Graduate School of Engineering, Osaka University, Japan (Supervisor Prof. Hiroshi Masuhara)&br;&br;Served ''Doctor of Philosophy in Engineering'' degree under the title of '''''“Processing and Manipulation of Organic and Protein Crystals in Solution by Femtosecond Laser Ablation”'''''&br;&br;Apr.- Jul. 2003 Guest Student, Materials group, Paul Scherrer Institut, Switzerland (Host: Dr. Thomas K. M. Lippert))&br;&br;2001 - 2003 Maser Thesis, Department of Applied Physics, Graduate School of Engineering, Osaka University, Japan&br;&br;1997 - 2001 Bachelor work, Department of Applied Science, School of Engineering, Osaka University, Japan|
**Peer-reviewed Papers [#i50c499f]
+"Protein crystallization with paper"
Miki Matsuoka, Keisuke Kakinouchi, Hiroaki Adachi, Mihoko Maruyama, Shigeru Sugiyama, Satoshi Sano, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Masashi Yoshimura, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Kazufumi Takano
'''Japanese Journal of Applied Physics (Rapid Communications)''', in press.&br;&br;
+"Quantitative Evaluation of Cancer Cell Adhesion to Self-Assembled Monolayer-Patterned Substrates by Reflection Interference Contrast Microscopy"
Takahisa Matsuzaki, Kosaku Ito, Kentaro Masuda, Eisuke Kakinuma, Rumi Sakamoto, Kentaro Iketaki, Hideaki Yamamoto, Masami Suganuma, Naritaka Kobayashi, Seiichiro Nakabayashi, Takashi Tanii, Hiroshi Y. Yoshikawa*
'''Journal of Physical Chemistry B''', ''120'' (2016) 1221-1227.&br;&br;
+"Growth of high-strength protein crystals with nanofibers"
Miki Matsuoka, Keisuke Kakinouchi, Hiroaki Adachi, Mihoko Maruyama, Shigeru Sugiyama, Iori Nakabayashi, Hiroshi Tsuchikura, Atsushi Kuwahara, Satoshi Sano, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Masashi Yoshimura, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Kazufumi Takano
'''Applied Physics Express''', 9 (2016) 035503 (3 pages).&br;&br;
+"Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell-Driven Condensation"
Takanori Takebe*, Masahiro Enomura, Emi Yoshizawa, Masaki Kimura, Hiroyuki
Koike, Yasuharu Ueno, Takahisa Matsuzaki, Takashi Yamazaki, Takafumi
Toyohara, Kenji Osafune, Hiromitsu Nakauchi, Hiroshi Y. Yoshikawa*(corresponding author), and
Hideki Taniguchi
'''Cell Stem Cell''', ''16'' (2015) 556-565 ''(Selected as a front cover)''.
''This article was featured in Nature Methods, 12 (2015) 600.''&br;&br;
+"Development of protein seed crystals reinforced with high-strength hydrogels"
Shigeru Sugiyama, Noriko Shimizu, Keisuke Kakinouchi, Osamu Hiraoka,Hiroyoshi Matsumura, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Mihoko Maruyama, Masashi Yoshimura, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Michio Murata and Yusuke Mori
'''ChemEngComm''', ''17'' (2015) 8064-8071.&br;&br;
+"A crystallization technique for obtaining large protein crystals with increased mechanical stability using agarose gel combined with a stirring technique"
Mihoko Maruyama, Yuki Hayashi, Hiroshi Y. Yoshikawa, Shino Okada, Haruhiko Koizumi, Masaru Tachibana, Shigeru Sugiyama, Hiroaki Adachi, Hiroyoshi Matsumura, Tsuyoshi Inoue, Kazufumi Takano, Satoshi Murakami, Masashi Yoshimura and Yusuke Mori
'''Journal of Crystal Growth''', in press.&br;&br;
+"Live Cell Tracking of Symmetry Break in Actin Cytoskeleton Triggered by Abrupt Changes in Micromechanical Environments"
S. Inoue, M. Hörning, V. Frank, S. Kaufmann, H.Y. Yoshikawa, J.P. Madsen, A.L. Lewis, S.P. Armes and M. Tanaka
'''Biomaterials Science ''', ''3'' (2015) 1539-1544.&br;&br;
+"Selective crystallization of metastable phase of acetaminophen by ultrasonic irradiation"
Yoichiro Mori, Mihoko Maruyama, Yoshinori Takahashi, Kenji Ikeda, Suguru Fukukita, Hiroshi Y. Yoshikawa, Shino Okada, Hiroaki Adachi, Shigeru Sugiyama, Kazufumi Takano, Satoshi Murakami, Hiroyoshi Matsumura, Tsuyoshi Inoue, Masashi Yoshimura, Yusuke Mori
'''Applied Physics Express''', ''8'' (2015) 045501 (5 pages).&br;&br;
+"Spiral growth can enhance both the normal growth rate and quality of tetragonal lysozyme crystals grown under a forced solution flow"
Hayashi, Yuki; Maruyama, Mihoko; Yoshimura, Masashi; Okada, Shino; Yoshikawa, Hiroshi; Sugiyama, Shigeru; Adachi, Hiroaki; Matsumura, Hiroyoshi; Inoue, Tsuyoshi; Takano, Kazufumi; Murakami, Satoshi; Mori, Yusuke
'''Crystal Growth & Design''', ''15'', (2015) 2137-2143.&br;&br;
+"Quantifying Adhesion Mechanisms and Dynamics of Human Hematopoietic Stem Cells and Progenitor Cells"
Alexandra Burk, Cornelia Monzel, Hiroshi Y. Yoshikawa, Patrick Wuchter, Rainer Saffrich, Volker Eckstein, Motomu Tanaka, and Anthony D. Ho
'''Scientific Reports''', ''5'' (2015) 9370 (8 pages). &br;&br;
+“Selective crystallization of the metastable phase of indomethacin at the interface of liquid/air bubble induced by the femtosecond laser irradiation"
Kenji Ikeda, Mihoko Maruyama, Yoshinori Takahashi, Yoichiro Mori, Hiroshi Y. Yoshikawa, Shino Okada, Hiroaki Adachi, Shigeru Sugiyama, Kazufumi Takano, Satoshi Murakami, Hiroyoshi Matsumura, Tsuyoshi Inoue, Masashi Yoshimura, and Yusuke Mori
'''Applied Physics Express''', ''8'' (2015) 045501 (5 pages). ''(Selected as SPOTLIGHTS)''
''This article was featured in Research Highlights JSAP Bulletin, 1 (2015).[[Link>http://jsap-bulletin.jsap.or.jp/en/research_highlights/vol1_3]]''&br;&br;
+“Cytoadhesion of P. falciparum-Infected Erythrocytes to Chondroitin-4-Sulfate is Cooperative and Shear- Enhanced”
H. Rieger, H. Y. Yoshikawa, K. Quadt, C. P. Sanchez, M. Tanaka, and M. Lanzer
'''Blood''', ''125'' (2015) 383-391.&br;&br;
+"A new practical technique for high quality protein crystallization with the solution stirring technique at the interface between high-concentrated hydrogel and solution"
Y. Aoki, M. Maruyama, Y. Takahashi, M. Yoshimura, H. Y. Yoshikawa, S. Sugiyama, H. Adachi, K. Takano, S. Murakami, H. Matsumura, T. Inoue, Y. Mori
'''Japanese Journal of Applied Physics''', ''53'' (2014) 065502.&br;&br;
+"High Contrast Visualization of Cell-Hydrogel Contact by Advanced Interferometric Optical Microscopy"
T. Matsuzaki, G. Sazaki, M. Suganuma, T. Watanabe, T. Yamazaki, M. Tanaka, S. Nakabayashi, H. Y. Yoshikawa(corresponding author)
'''Journal of Physical Chemistry Letters''', ''5'' (2014) 253–257.&br;&br;
+"Laser ablation for protein crystal nucleation and seeding"
H. Y. Yoshikawa (corresponding author), R. Murai, H. Adachi, S. Sugiyama, M. Maruyama, Y. Takahashi, K. Takano, H. Matsumura, T. Inoue, S. Murakami, H. Masuhara, and Y. Mori
'''Chemical Society Reviews (IF = 24.892)''', ''43'' (2014) 2147-2158.&br;&br;
+"Reconstruction of joint hyaline cartilage by autologous progenitor cells derived from ear elastic cartilage"
Mitsuru Mizuno, Shinji Kobayashi, Takanori Takebe, Hiroomi Kan, Yuichiro Yabuki, Takahisa Matsuzaki, Hiroshi Y. Yoshikawa, Seiichiro Nakabayashi, Lee Jeong Ik, Jiro Maegawa, Hideki Taniguchi
'''Stem Cells''', ''32'' (2014) 816–821.&br;&br;
+"Hydrogen nanobubble at normal hydrogen electrode"
S. Nakabayashi, R. Shinozaki, Y. Senda and H. Y. Yoshikawa
'''Journal of Physics: Condensed Matter''', ''25'' (2013) 184008 (8 pages).&br;&br;
+"Morphology and Adhesion Strength of Myoblast Cells on Photocurable Gelatin under Native and Non-native Micromechanical Environments"
H. Y. Yoshikawa, T. Kawano, T. Matsuda, S. Kidoaki, M. Tanaka
'''Journal of Physical Chemistry B''', ''117'' (2013) 4081-4088.&br;&br;
+"Effects of Gel-Solution Interfaces on Femtosecond Laser-Induced Nucleation of Protein"
S. Nakayama, H. Y. Yoshikawa(corresponding author), R. Murai, M. Kurata, H. Maruyama, S. Sugiyama, Y. Aoki, Y. Takahashi, M. Yoshimura, S. Nakabayashi, H. Adachi, H. Matsumura, T. Inoue, K. Takano, S. Murakami, Y. Mori
'''Crystal Growth & Design''', ''13'' (2013) 1491-1496.&br;&br;
+"Magnetic field induced hopping among Arnold entrainment tongues of coupled iron electrochemical oscillators"
H. Koyama, H. Nakao, H. Y. Yoshikawa, S. Nakabayashi
'''Chemical Physics Letters''', ''555'' (2013) 268-273.&br;&br;
+"溶液流れ下におけるタンパク質の結晶成長"
丸山美帆子，中村真利子，佐崎 元，高橋義典，吉村政志，吉川洋史，杉山成，安達宏昭，松村浩由，井上豪，高野和文，村上聡，森 勇介
'''日本結晶成長学会誌'''，''40'' (2013) 72-82.&br;&br;
+"Spatially Precise, Soft Microseeding of Single Protein Crystals by Femtosecond Laser Ablation"
H. Y. Yoshikawa, Y. Hosokawa, R. Murai, G. Sazaki, T. Kitatani, H. Adachi, T. Inoue, H. Matsumura, K. Takano, S. Murakami, S. Nakabayashi, Y. Mori, and H. Masuhara
'''Crystal Growth & Design''', ''12'' (2012) 4334-4339.&br;&br;
+"Quantitative Evaluation of Adhesion of Osteosarcoma Cells to Hydrophobic Polymer Substrate with Tunable Elasticity"
H. Y. Yoshikawa, J. Cui, K. Kratz, T. Matsuzaki, S. Nakabayashi, A. Marx, U. Engel, A. Lendlein, and M. Tanaka
'''Journal of Physical Chemistry B''', ''116'' (2012) 8024-8030.&br;&br;
+" Effects of a forced solution flow on the step advancement on {110} faces of tetragonal lysozyme crystals: direct visualization of individual steps under a forced solution flow"
M. Maruyama, H. Kawahara, G. Sazaki, S. Maki, Y. Takahashi, H. Y. Yoshikawa, S. Sugiyama, H. Adachi, K. Takano, H. Matsumura, T. Inoue, S. Murakami, and Y. Mori
'''Crystal Growth & Design''', ''12'' (2012) 2856-2863.&br;&br;
+"Influence of Energy and Wavelength on Femtosecond Laser-Induced Nucleation of Protein"
R. Murai, H. Y. Yoshikawa (Corresponding Author, First two authors contributed equally), H. Hasenaka, Y. Takahashi, M. Maruyama, S. Sugiyama, H. Adachi, K. Takano, H. Matsumura, S. Murakami, T. Inoue, Y. Mori
'''Chemical Physics Letters''', ''510'' (2011) 139-142.&br;&br;
+“Physical Chemistry of Biological Interfaces: Generic and Specific Roles of Soft Interlayers”
M. Tanaka, E. Schneck, H. Y. Yoshikawa, F. F. Rossetti
'''Chemistry -An Asian Journal-''', ''6'' (2011) 1728-1738.&br;&br;
+“Quantitative Evaluation of Mechanosensing of Cells on Dynamically Tunable Hydrogels”
H. Y. Yoshikawa, F. F. Rossetti, S. Kaufmann, T. Kaindl, J. Madsen, U. Engel, A. L. Lewis, S. P. Armes, and M. Tanaka
'''Journal of the American Chemical Society''', ''133'' (2011) 1367-1374.
This article was featured in the press release of Univ. Heidelberg.
http://www.uni-heidelberg.de/presse/news2011/pm20110726_zelladhaesion.html &br;&br;
+"フェムト秒レーザー誘起キャビテーションバブルによるタンパク質の結晶核発生"
吉川 洋史，村井 良多，杉山 成，安達 宏昭，松村 浩由，井上 豪，村上 聡，高野 和文，森 勇介
'''日本結晶成長学会誌''', ''28'' (2011) 161-168.
(Cover article)&br;&br;
+”Enhancement of Femtosecond Laser-Induced Nucleation of Protein in a Gel Solution”
R. Murai, H. Y. Yoshikawa (Corresponding Author), Y. Takahashi, M. Maruyama, S. Sugiyama, G. Sazaki, H. Adachi, K. Takano, H. Matsumura, S. Murakami, T. Inoue, and Y. Mori
'''Applied Physics Letters''', ''96'' (2010) 043702-043704.
Selected for the Virtual Journal of Biological Physics Research, February 1, 2010&br;&br;
+“Growth of large protein crystals by top-seeded solution growth together with floating and solution-stirring technique”
N. Shimizu, S. Sugiyama, M. Maruyama, H. Y. Yoshikawa, Y. Takahashi, H. Adachi, K. Takano, S. Murakami, T. Inoue, H. Matsumura, and Y. Mori
'''Crystal Growth & Design''', ''9'' (2009) 5227-5232.&br;&br;
+“Femtosecond Laser-Induced Nucleation of Protein in Agalose Gel”
H. Y. Yoshikawa, R. Murai, S. Sugiyama, G. Sazaki, T. Kitatani, Y. Takahashi, H. Adachi, H. Matsumura, S. Murakami, T. Inoue, K. Takano, and Y. Mori,
'''Journal of Crystal Growth''', ''311'' (2009) 956-959.&br;&br;
+“Laser Energy Dependence on Femtosecond Laser-Induced Nucleation of Protein”
H. Y. Yoshikawa, R. Murai, S. Maki, T. Kitatani, S. Sugiyama, G. Sazaki, H. Adachi, T. Inoue, H. Matsumura, K. Takano, S. Murakami, T. Sasaki, and Y. Mori
'''Applied Physics A''', ''93'' (2008) 911-915.&br;&br;
+“Effect of Solution Flow Produced by Rotary Shaker on Protein Crystallization”
R. Murai, H. Y. Yoshikawa, H. Kawahara, S. Maki, S. Sugiyama, T. Kitatani, H. Adachi, K. Takano, H. Matsumura, S. Murakami, T. Inoue, T. Sasaki, and Y. Mori
'''Journal of Crystal Growth''', ''310'' (2008) 2168-2172.&br;&br;
+“Protein Crystallization in a 100 nl Solution with New Stirring Equipment”
S. Maki, R. Murai, H. Y. Yoshikawa, T. Kitatani, S. Nakata, H. Kawahara, H. Hasenaka, A. Kobayashi, S. Okada, S. Sugiyama, H. Adachi, H. Matsumura, K. Takano, S. Murakami, T. Inoue, T. Sasaki and Y. Mori
'''Journal of Synchrotron Radiation''', ''15'' (2008) 269-272.&br;&br;
+“New Technique of Manipulating a Protein Crystal Using Adhesive Material”
T. Kitatani, S. Sugiyama, H. Matsumura, H. Adachi, H. Y. Yoshikawa, S. Maki, S. Murakami, T. Inoue, Y. Mori, and K. Takano
'''Applied Physics Express''', ''1'' (2008) 037002-037004.&br;&br;
+“Crystallization and Preliminary X-ray Crystallographic Analysis of Ca2+-free Primary Ca2+-sensor of Na+/Ca2+ Exchanger”
M. Mima, C. Kawai, K. Paku, K. Tomoo, T. Ishida, S. Sugiyama, H. Matsumura, T. Kitatani, H. Y. Yoshikawa, S. Maki, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori, S. Kita, and T. Iwamoto
'''Acta Crystallographica F''', ''64'' (2008) 1125-1127.&br;&br;
+“Crystallization and Preliminary Neutron Diffraction Studies of HIV-1 Protease Cocrystallized with Inhibitor KNI-272”
H. Matsumura, M. Adachi, S. Sugiyama, S. Okada, M. Yamakami, T. Tamada, K. Hidaka, Y. Hayashi, T. Kimura, Y. Kiso, T. Kitatani, S. Maki, H. Y. Yoshikawa, H. Adachi, K. Takano, S. Murakami, T. Inoue, R. Kuroki, and Y. Mori
'''Acta Crystallographica F''', ''64'' (2008) 1003-1006.&br;&br;
+”Femtosecond Laser-induced Crystallization of Protein in Gel Medium”
K. Nakamura, Y. Sora, H. Y. Yoshikawa, Y. Hosokawa, R. Murai, H. Adachi Y. Mori, T. Sasaki, and H. Masuhara
'''Applied Surface Science''', ''253'' (2007) 6425-6429.&br;&br;
+“Spatial Control of Urea Crystal Growth by Focused Femtosecond Laser Irradiation”
H. Y. Yoshikawa, Y. Hosokawa, and H. Masuhara
'''Crystal Growth & Design''', ''6'' (2006) 302-305.&br;&br;
+“Explosive Crystallization of Urea Triggered by Focused Femtosecond Laser Irradiation”
H. Y. Yoshikawa, Y. Hosokawa, and H. Masuhara
'''Japanese Journal of Applied Physics''', 45 (2006) L23-L26.&br;&br;
+“Fluorescence Evaluation of Antigen-Antibody Reactivity on Surface of Proteinaceous Occlusion Body: Toward Application in Reusable Protein Chip”
H. Y. Yoshikawa, Y. Hosokawa, K. Suzuki, K. Ikeda, H. Mori, and H. Masuhara
'''Japanese Journal of Applied Physics''', ''45'' (2006) 323-327.&br;&br;
+「フェムト秒レーザーアブレーションによる尿素の結晶化」
吉川洋史、細川陽一郎、増原宏
レーザ加工学会誌, Vol. 13 (2006) 147-151.&br;&br;
+”Synthesis and Characterization of New Hard Polyurethanes with Triazene Pendants”
E. C. Buruiana, V. Melinte, T. Bruiana, T. Lippert, H. Yoshikawa, and H. Masuhara
'''Journal of Photochemistry and Photobiology A: Chemistry''', ''171'' (2005) 261-267.&br;&br;
+“Femtosecond Laser Ablation Transfer and Phase Transition of Phthalocyanine Solids”
T. Asahi, H. Y. Yoshikawa, M. Yashiro, and H. Masuhara
'''Applied Surface Science''', ''197-198'' (2002) 777-781.&br;&br;
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