業績
 

原著論文

1. Takaomi Sasaki, Saya Takita, Takashi Fujishiro, Yunosuke Shintani, Satoki Nojiri, Ryota Yasui, Tetsuro Yonesaki, Yuichi Otsuka "Phage single-stranded DNA-binding protein or host DNA damage triggers the activation of the AbpAB phage defense system" mSphere, 2023, accepted.


2. Takashi Fujishiro, Kyosei Takaoka "Class III hybrid cluster protein homodimeric architecture shows evolutionary relationship with Ni, Fe-carbon monoxide dehydrogenases" Nat. Commun. 2023, 14, 5609.


3. Ryosuke Nakamura, Shoko Ogawa, Yasuhiro Takahashi, Takashi Fujishiro "Cycloserine enantiomers inhibit PLP-dependent cysteine desulfurase SufS via distinct mechanisms" FEBS J. 2022, 289(19), 5947-5970.


4. Takashi Fujishiro, Miho Ooi, Kyosei Takaoka, "Crystal structure of Escherichia coli class II hybrid cluster protein, HCP, reveals a [4Fe-4S] cluster at the N-terminal protrusion" FEBS J. 2021, 288(23), 6752-6768.


5. Kouhei Kunichika, Ryosuke Nakamura, Takashi Fujishiro, Yasuhiro Takahashi, "The structure of the dimeric state of IscU harboring two adjacent [2Fe-2S] clusters provides mechanistic insights into cluster conversion to [4Fe-4S]" Biochemistry, 2021, 60, 1569-1572.


6. Hitoshi Nakamoto, Yuhei Yokoyama, Takahiro Suzuki, Yuri Miyamoto, Takashi Fujishiro, Masaaki Morikawa, Yoshihiko Miyata, "A cyclic lipopeptide sufractin is a species-selective Hsp90 inhibitor that suppresses cyanobacterial growth" J. Biochem., 2021, 170, 255-264.


7. Takashi Fujishiro, Shoko Ogawa, "The nickel-sirohydrochlorin formation mechanism of the ancestral class II chelatase CfbA in coenzyme F430 biosynthesis" Chem. Sci., 2021, 12, 2172-2180.


8. Sakiko Sato, Yumeka Matsushima, Miaki Kanazawa, Naoyuki Tanaka, Takashi Fujishiro, Kouhei Kunichika, Ryosuke Nakamura, Hiroaki Tomioka, Kei Wada, Yasuhiro Takahashi, "Evidence for dynamic in vivo interconversion of the conformational states of IscU during iron-sulfur cluster biosynthesis" Mol. Microbiol., 2021, 115, 807-818.


9. Ryosuke Nakamura, Masahide Hikita, Shoko Ogawa, Yasuhiro Takahashi, Takashi Fujishiro, "Snapshots of PLP-substrate and PLP-product external aldimines as intermediates in two types of cysteine desulfurase enzymes" FEBS J. 2020, 287, 1138-1154.


10. Naoyuki Tanaka, Eiki Yuda, Takashi Fujishiro, Kei Hirabayashi, Kei Wada, Yasuhiro Takahashi, "Identification of IscU residues critical for de novo iron-sulfur cluster assembly" Mol. Microbiol. 2019, 112, 1769–1783.


11. Takashi Fujishiro, Yukino Shimada, Ryosuke Nakamura, Miho Ooi, "Structure of sirohydrochlorin ferrochelatase SirB: The last of the structures of the class II chelatase family" Dalton Trans., 2019, 48, 6083–6090.


12. Nao Yokoyama, Chihiro Nonaka, Yukari Ohashi, Masaharu Shioda, Takuya Terahata, Wen Chen, Kotomi Sakamoto, Chihiro Maruyama, Takuya Saito, Eiki Yuda, Naoyuki Tanaka, Takashi Fujishiro, Tomohisa Kuzuyama, Kei Asai, and Yasuhiro Takahashi, "Distinct roles for U-type proteins in iron-sulfur cluster biosynthesis revealed by genetic analysis of the Bacillus subtilis sufCDSUB operon" Mol. Microbiol. 2018, 107, 688–703.


13. Takashi Fujishiro, Takuya Terahata, Kouhei Kunichika, Nao Yokoyama, Chihiro Maruyama, Kei Asai, and Yasuhiro Takahashi, "Zinc-ligand swapping mediated complex formation and sulfur transfer between SufS and SufU for iron-sulfur cluster biogenesis in Bacillus subtilis" J. Am. Chem. Soc. 2017, 139, 18464–18467.


14. Eiki Yuda, Naoyuki Tanaka, Takashi Fujishiro, Nao Yokoyama, Kei Hirabayashi, Keiichi Fukuyama, Kei Wada, and Yasuhiro Takahashi, "Mapping the key residues of SufB and SufD essential for biosynthesis of iron-sulfur clusters" Sci. Rep. 2017, 7, article number:9387


15. Liping Bai, Takashi Fujishiro, Gangfeng Huang, Jürgen Koch, Atsushi Takabayashi, Makio Yokono, Ayumi Tanaka, Tao Xu, Xile Hu, Ulrich Ermler, and Seigo Shima, “Towards artificial methanogenesis: biosynthesis of the [Fe]-hydrogenase cofactor and characterization of the semisynthetic hydrogenase”, Faraday Discuss. 2017, 198, 37–58.


16. Takashi Fujishiro, Liping Bai, Tao Xu, Xiulan Xie, Michael Schick, Jörg Kahnt, Michael Rother, Xile Hu, Ulrich Ermler, and Seigo Shima, “Identification of HcgC as SAM-dependent pyridinol methyltransferase in [Fe]-hydrogenase cofactor biosynthesis” Angew. Chem. Int. Ed., 2016, 55, 9648–9651.


17. Osami Shoji, Takashi Fujishiro, Kousuke Nishio, Yukiko Kano, Hiroshi Kimoto, Shih-Cheng Chien, Hiroki Onoda, Atsushi Muramatsu, Shota Tanaka, Ayumi Hori, Hiroshi Sugimoto, Yoshitsugu Shiro, and Yoshihito Watanabe, “A substrate-binding-site mimic of H2O2-dependent cytochrome P450 produced by one-point mutagenesis and peroxygenation of non-native substrates” Catal. Sci. Technol., 2016, 6, 5806–5811.


18. Seigo Shima, Dafa Chen, Tao Xu, Matthew D. Wodrich, Takashi Fujishiro, Katherine M. Schultz, Jörg Kahnt, Kenichi Ataka, and Xile Hu, “Reconstitution of [Fe]-hydrogenase using model complexes” Nat. Chem., 2015,7, 995–1002.


19. Takashi Fujishiro, Kenichi Ataka, Ulrich Ermler, and Seigo Shima, “Towards a functional identification of catalytically inactive [Fe]-hydrogenase paralogs” FEBS J., 2015, 282, 3412–3423.


20. Takashi Fujishiro, Jörg Kahnt, Ulrich Ermler, and Seigo Shima, “Protein-pyridinol thioester precursor for biosynthesis of the organometallic acyl-iron ligand in [Fe]-hydrogenase cofactor” Nat. Commun., 2015, 6, article number:6895.


21. Takashi Fujishiro, Ulrich Ermler, and Seigo Shima, “A possible iron delivery function of the dinuclear iron center of HcgD in [Fe]-hydrogenase cofactor biosynthesis” FEBS Lett., 2014, 588, 2789–2793.


22.Takashi Fujishiro, Haruka Tamura, Michael Schick, Jörg Kahnt, Xiulan Xie, Ulrich Ermler, and Seigo Shima, “Identification of the HcgB enzyme in [Fe]-hydrogenase-cofactor biosynthesis” Angew. Chem Int. Ed., 2013, 52, 12555-12558.


23. Haruka Tamura, Marco Salomone-Stagni, Takashi Fujishiro, Eberhard Warkentin, Wolfram Meyer-Klaucke, Ulrich Ermler and Seigo Shima, “Crystal structure of [Fe]-hydrogenase in complex with inhibitory isocyanides: implications for the H2-activation site.” Angew. Chem Int. Ed., 2013, 52, 9656-9659.


24. Takashi Fujishiro, Osami Shoji, Norifumi Kawakami, Takahiro Watanabe, Hiroshi Sugimoto, Yoshitsugu Shiro, and Yoshihito Watanabe, “Chiral-substrate-assisted stereoselective epoxidation catalyzed by H2O2-dependent cytochrome P450SPα” Chem. Asian J. 2012, 7, 2286–2293.


25. Jiakun Xu, Osami Shoji, Takashi Fujishiro, Takahiro Ohki, Takafumi Ueno, and Yoshihito Watanabe, “Construction of biocatalysts using the myoglobin scaffold for the synthesis of indigo from indole” Catal. Sci. Technol. 2012, 2, 739-744.


26. Takashi Fujishiro, Osami Shoji, Shingo Nagano, Hiroshi Sugimoto, Yoshitsugu Shiro, and Yoshihito Watanabe, “Crystal structure of H2O2-dependent cytochrome P450SPα with its bound fatty acid substrate: Insight into the regioselective hydroxylation of fatty acids at the alpha position” J. Biol. Chem. 2011, 286, 29941-29950.


27. Takashi Fujishiro, Osami Shoji, and Yoshihito Watanabe, “Non-covalent modification of the active site of cytochrome P450 for inverting the stereoselectivity of monooxygenation” Tetrahedron Lett., 2011, 52, 395–397.


28. Osami Shoji, Takashi Fujishiro, Shingo Nagano, Shota Tanaka, Takuya Hirose, Yoshitsugu Shiro, and Yoshihito Watanabe, “Understanding substrate misrecognition of hydrogen peroxide dependent cytochrome P450 from Bacillus subtilis” J. Biol. Inorg. Chem., 2010, 15, 1331–1339.


29. Osami Shoji, Christian Wiese, Takashi Fujishiro, Chikako Shirataki, Bernhard Wünsch, and Yoshihito Watanabe, “Aromatic C-H Bond Hydroxylation by P450 Peroxygenases: A Facile Colorimetric Assay for Monooxygenation Activities of Enzymes Based on the Russig’s Blue Formation” J. Biol. Inorg. Chem., 2010, 15, 1109–1115.


30. Osami Shoji, Takashi Fujishiro, Hiroshi Nakajima, Misa Kim, Shingo Nagano, Yoshitsugu Shiro, and Yoshihito Watanabe, “Hydrogen Peroxide Dependent Monooxygenations by Tricking the Substrate Recognition of Cytochrome P450BSβ” Angew. Chem. Int. Ed., 2007, 46, 3656-3659.

総説、 Proceedings、著書など

総説


1. Takashi Fujishiro, Ryosuke Nakamura, Kouhei Kunichika, Yasuhiro Takahashi "Structural diversity of cysteine desulfurases involved in iron-sulfur cluster biosynthesis" Biophys. Physicobiol. 2022, 19, e190001.


2. 藤城貴史,* 中村亮裕, 高橋康弘* 「鉄硫黄クラスター生合成系の硫黄供給酵素群:共通構造と機能分化・多様性」、 生物物理、2021年6月号(3号) vol.61, 180-182.


  1. 3.嶋盛吾* , 藤城貴史「[Fe]-ヒドロゲナーゼの活性中心コファクターと立体構造解析に基づく

   その生合成酵素の同定」、酵素工学ニュース(酵素工学研究会誌), 2016年10月号, vol.76, pp.26-30.


  1. 4.藤城貴史, 嶋盛吾 「酵素立体構造からの機能解読」、生物工学会誌, 2014年12月号, vol.92, p.676.




Book chapter


1. Takashi Fujishiro, "Escherichia coli class II hybrid cluster protein, HCP" In Encyclopedia of Inorganic and Bioinorganic Chemistry, R.A. Scott (Ed.). 2023.



2. Takashi Fujishiro, "Sirohydrochlorin nickelochelatase CfbA" In Encyclopedia of Inorganic and Bioinorganic Chemistry, R.A. Scott (Ed.). 2022.



3. Wolfgang Weigand, Ulf-Peter Apfel, Marius Horch, Lenz Zebger and Takashi Fujishiro "Part I: chapter 2 Hydrogen development" Bioorganometallic Chemistry, (Edited by Prof. Dr. Wolfgang Weigand and Dr. Ulf-Peter Apfel), De Gruyter, , pp.106– 136, 2020.


4. Seigo Shima, Takashi Fujishiro, and Ulrich Ermler "Structure and function of

    [Fe]-hydrogenase and biosynthesis of the FeGP cofactor" Biohydrogen, (Edited by Prof. Dr. Matthias Rögner), De Gruyter, 2015, chapter 6 , pp. 127-144.




Proceedings


1.Takashi Fujishiro, Haruka Tamura, Michael Schick, Jörg Kahnt, Xiulan Xie, Ulrich Ermler, Seigo Shima “Identification of the Hcg enzymes in biosynthesis of [Fe]-hydrogenase cofactor by a structural genomics-based approach”, J. Biol. Inorg. Chem., 2014, vol.19, p.S767 (EuroBIC12).



2.Takashi Fujishiro, Haruka Tamura, Michael Schick, Jörg Kahnt, Xiulan Xie, Ulrich Ermler, Seigo Shima “Biochemical and X-ray crystal structure analyses of Hcg proteins involved in biosynthesis of the FeGP cofactor of [Fe]-hydrogenase”, J. Biol. Inorg. Chem., 2014, vol.19, p.S223. (ICBIC16).




その他


1.Maya Murata, Taichi Murakami, Eiki Yuda, Nanami Mukai, Xintong Zheng, Natsumi Kurachi, Sachiko Mori, Shoko Ogawa, Kouhei Kunichika, Takashi Fujishiro, Kei Wada, Yasuhiro Takahashi " The minimal SUF system can substitute for the canonical iron-sulfur cluster biosynthesis systems by using inorganic sulfide as the sulfur source."bioRxiv 2024.03.20.586028; doi: https://doi.org/10.1101/2024.03.20.586028



2.藤城貴史,“埼玉新聞サイ・テクこらむ–金属と生命、そして進化” 2019年10月9日 埼玉新聞朝刊掲載.



3. 藤城貴史, 埼玉大学研究機構科学分析支援センター機関誌CACS Forum (Comprehensive analysis center for Science) Forum紀要, 「生体由来金属クラスター生合成の生物無機化学」、No.9, 2018.12.



4. 藤城貴史, Chem-Station第133回スポットライトリサーチ

    「システインから無機硫黄を取り出す酵素反応の瞬間を捉える」, 2018年1月記事

    URL: https://www.chem-station.com/blog/2018/01/sufssufsu.html


5. 藤城貴史, "フロンティア生命化学研究会レターNo.55(2017, October)留学体験記

   「マックスプランク陸生微生物学研究所留学体験記〜化学から生物学へ〜」" 2017年10月号.


6. 藤城貴史,“埼玉新聞サイ・テクこらむ–タンパク質の構造と役割” 2016年2月10日 埼玉新聞朝刊掲載.


7. 藤城貴史, 「デコイ分子によるヘム酵素の基質選択性の変換」, Bull. Jpn. Soc. Coord. Chem.

   2008, 51, 82–84.

主な著書・論文

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