業績
 

原著論文

61. Nakamura, R., Ogawa, S., Takahashi, Y., Fujishiro, T. Cycloserine enantiomers inhibit PLP-dependent cysteine desulfurase SufS via distinct mechanisms. FEBS J. in press.

60. Kunichika, K., Nakamura, Y., Fujishiro, T., Takahashi, Y. The structure of the dimeric state of IscU Harboring two adjacent [2Fe–2S] clusters provides mechanistic insights into cluster conversion to [4Fe–4S]. (2021) Biochemistry, 60, 1569-1572.

59. Sato, S., Matsushima, Y., Kanazawa, M., Tanaka, N., Fujishiro, T., Kunichika, K., Nakamura, R., Tomioka, H., Wada, K., Takahashi, Y. Evidence for dynamic in vivo interconversion of the conformational states of IscU during iron–sulfur cluster biosynthesis. (2021) Mol. Microbiol. 115, 807-818.

58. Nakamura, R., Hikita, M., Ogawa, S., Takahashi, Y., Fujishiro, T. Snapshots of PLP-substrate and PLP-product  external aldimines as intermediates in two types of cysteine desulfurase enzymes. (2020) FEBS J. 287, 1138-1154.

57. Tanaka, N., Yuda, E., Fujishiro, T., Hirabayashi, K., Wada, K., Takahashi, Y. Identification of IscU residues critical for de novo iron‐sulfur cluster assembly. (2019) Mol. Microbiol. 112, 1769-1783.

56. Yokoyama, N., Nonaka, C.,  Ohashi, Y., Shioda, M., Terahata, T., Chen, W., Sakamoto, K., Maruyama, C., Saito, T., Yuda, E., Tanaka, N., Fujishiro, T., Kuzuyama, T., Asai, K., Takahashi, Y. (2018) Distinct roles for U-type proteins in iron-sulfur cluster biosynthesis revealed by genetic analysis of the Bacillus subtilis sufCDSUB operon. Mol. Microbiol., 107(6), 688–703.

55. Fujishiro, T., Terahata, T., Kunichika, K., Yokoyama, N., Maruyama, C., Asai, K., Takahashi, Y. (2017) Zinc-ligand swapping mediated complex formation and sulfur transfer between SufS and SufU for iron-sulfur cluster biogenesis in Bacillus subtilis. J. Am. Chem. Soc., 139, 18464–18467.

54. Yuda, E., Tanaka, N., Fujishiro, T., Yokoyama, N., Hirabayashi, K., Fukuyama, K., Wada, K., Takahashi, Y. (2017) Mapping the key residues of SufB and SufD essential for biosynthesis of iron-sulfur clusters. Sci. Rep. 7, article number: 9387.

53. Takao, H., Hirabayashi, K., Nishigaya, Y., Kouriki, H., Nakaniwa, T., Hagiwara, Y., Harada, J., Sato, H., Yamazaki, T., Sakakibara, Y., Suiko, M., Asada, Y., Takahashi, Y., Yamamoto, K., Fukuyama, K., Sugishima, M., Wada, K. (2017) A substrate-bound structure of cyanobacterial biliverdin reductase identifies stacked substrates as critical for activity. Nat. Commun. 8, article number: 14397.

52. Tanaka, N., Kanazawa, M., Tonosaki, K., Yokoyama, N., Kuzuyama, T., Takahashi, Y. (2016) Novel features of the ISC machinery revealed by characterization of Escherichia coli mutants that survive without iron–sulfur clusters. Mol. Microbiol. 99, 835-848.

51. Hirabayashi, K., Yuda, E., Tanaka, N., Katayama, S., Iwasaki, K., Matsumoto, T., Kurisu, G., Outten, F.W., Fukuyama, K., Takahashi, Y., Wada, K. (2015) Functional dynamics revealed by the structure of the SufBCD Complex, a novel ATP-binding cassette (ABC) protein that serves as a scaffold for iron-dulfur cluster biogenesis. J. Biol. Chem. 290, 29717–29731.

50. Okutani, S., Iwai, T., Iwatani, S., Matsuno, K., Takahashi, Y., Hase, T. (2015) Response of Fe-S cluster assembly machinery of Escherichia coli to mechanical stress in a model of amino-acid crystal fermentation. J. Biosci. Bioengineer. 120(3), 287-293.

49. Nakai, T., Ito, H., Kobayashi, K. Takahashi, Y., Hori, H., Tsubaki, M., Tanizawa, K., Okajima, T. (2015) The radical S-adenosyl-L-methionine enzyme QhpD catalyzes sequential formation of intra-protein sulfur-to-methylene carbon thioether bonds. J. Biol. Chem. 290, 11144-11166.

48. Otsuka, Y., Miki, K., Koga, M., Katayama, N., Morimoto, W., Takahashi, Y., Yonesaki T. (2010) IscR regulates RNase LS activity by repressing rnlA transcription. Genetics 185, 823-830.

47. Hagiwara, Y., Sugishima, M., Khawn, H., Kinoshita, H., Inomata, K., Shang, L., Lagarias, J.C., Takahashi, Y., and Fukuyama, K. (2010) Structural insights into vinyl reduction regiospecificity of phycocyanobilin:ferredoxin oxidoreductase (PcyA). J. Biol. Chem. 285, 1000-1007.

46. Nakajima H, Takatani N, Yoshimitsu K, Itoh M, Aono S, Takahashi Y, and Watanabe Y (2010) The role of the Fe-S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii. FEBS J. 277, 817–832.

45. Wada, K., Sumi, N., Nagai, R., Iwasaki, K., Sato, T., Suzuki, K., Hasegawa, Y., Kitaoka, S., Minami, Y., Outten, F.W., Takahashi, Y., and Fukuyama K. (2009) Molecular dynamism of Fe-S cluster biosynthesis implicated by the structure of the SufC2-SufD2 complex. J. Mol. Biol., 387, 245-258.

44. Shimomura, Y., Wada, K., Fukuyama, K., and Takahashi Y. (2008) The asymmetric trimeric architecture of [2Fe-2S] IscU: implications for its scaffolding during iron-sulfur cluster biosynthesis. J. Mol. Biol., 383, 133-143.

43. Dey, A., Jenney, F. E., Jr., Adams, M. W., Babini, E., Takahashi, Y., Fukuyama, K., Hodgson, K. O., Hedman, B., and Solomon, E. I. (2007) Solvent tuning of electrochemical potentials in the active sites of HiPIP versus ferredoxin., Science, 318, 1464-1468.

42. Shimomura, Y., Kamikubo, H., Nishi, Y., Masako, T., Kataoka, M., Kobayashi, Y., Fukuyama, K., and Takahashi, Y. (2007) Characterization and crystallization of an IscU-type scaffold protein with bound [2Fe-2S] cluster from the hyperthermophile, Aquifex aeolicus., J. Biochem. 142, 577-586.

41. Watanabe, S., Kita, A., Kobayashi, K., Takahashi, Y., and Miki, K. (2006) Crystallization and preliminary X-ray crystallographic studies of the oxidative-stress sensor SoxR and its complex with DNA., Acta Crystallogr. Sect. F 62, 1275-1277.

40. Hagiwara, Y., Sugishima, M., Takahashi, Y., and Fukuyama, K. (2006) Induced-fitting and electrostatic potential change of PcyA upon substrate binding demonstrated by the crystal structure of the substrate-free form., FEBS Lett. 580, 3823-3828.

39. Kitaoka, S., Wada, K., Hasegawa, Y., Minami, Y., Fukuyama, K., and Takahashi, Y. (2006) Crystal structure of Escherichia coli SufC, an ABC-type ATPase component of the SUF iron-sulfur cluster assembly machinery., FEBS Lett. 580, 137-143.

38. Hagiwara, Y., Sugishima, M., Takahashi, Y., and Fukuyama, K. (2006) Crystal structure of phycocyanobilin:ferredoxin oxidoreductase in complex with biliverdin IXalpha, a key enzyme in the biosynthesis of phycocyanobilin., Proc. Natl. Acad. Sci. U. S. A. 103, 27-32.

37. Wada, K., Hasegawa, Y., Gong, Z., Minami, Y., Fukuyama, K., and Takahashi, Y. (2005) Crystal structure of Escherichia coli SufA involved in biosynthesis of iron-sulfur clusters: implications for a functional dimer., FEBS Lett. 579, 6543-6548.

36. Shirakawa, T., Takahashi, Y., Wada, K., Hirota, J., Takao, T., Ohmori, D., and Fukuyama, K. (2005) Identification of variant molecules of Bacillus thermoproteolyticus ferredoxin: crystal structure reveals bound coenzyme A and an unexpected [3Fe-4S] cluster associated with a canonical [4Fe-4S] ligand motif., Biochemistry 44, 12402-12410.

35. Shimomura, Y., Takahashi, Y., Kakuta, Y., and Fukuyama, K. (2005) Crystal structure of Escherichia coli YfhJ protein, a member of the ISC machinery involved in assembly of iron-sulfur clusters., Proteins 60, 566-569.

34. Tokumoto, U., Kitamura, S., Fukuyama, K., and Takahashi, Y. (2004) Interchangeability and distinct properties of bacterial Fe-S cluster assembly systems: functional replacement of the isc and suf operons in Escherichia coli with the nifSU-like operon from Helicobacter pylori., J. Biochem. 136, 199-209.

33. Ali, V., Shigeta, Y., Tokumoto, U., Takahashi, Y., and Nozaki, T. (2004) An intestinal parasitic protist, Entamoeba histolytica, possesses a non-redundant nitrogen fixation-like system for iron-sulfur cluster assembly under anaerobic conditions., J. Biol. Chem. 279, 16863-16874.

32. Yano, T., Sklar, J., Nakamaru-Ogiso, E., Takahashi, Y., Yagi, T., and Ohnishi, T. (2003) Characterization of cluster N5 as a fast-relaxing [4Fe-4S] cluster in the Nqo3 subunit of the proton-translocating NADH-ubiquinone oxidoreductase from Paracoccus denitrificans., J. Biol. Chem. 278, 15514-15522.

31. Kriek, M., Peters, L., Takahashi, Y., and Roach, P. L. (2003) Effect of iron-sulfur cluster assembly proteins on the expression of Escherichia coli lipoic acid synthase., Protein Expr. Purif. 28, 241-245.

30. Takahashi, Y., and Tokumoto, U. (2002) A third bacterial system for the assembly of iron-sulfur clusters with homologs in archaea and plastids., J. Biol. Chem. 277, 28380-28383.

29. Mihara, H., Kato, S., Lacourciere, G. M., Stadtman, T. C., Kennedy, R. A., Kurihara, T., Tokumoto, U., Takahashi, Y., and Esaki, N. (2002) The iscS gene is essential for the biosynthesis of 2-selenouridine in tRNA and the selenocysteine-containing formate dehydrogenase H., Proc. Natl. Acad. Sci. U. S. A. 99, 6679-6683.

28. Tokumoto, U., Nomura, S., Minami, Y., Mihara, H., Kato, S., Kurihara, T., Esaki, N., Kanazawa, H., Matsubara, H., and Takahashi, Y. (2002) Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli., J. Biochem. 131, 713-719.

27. Kato, S., Mihara, H., Kurihara, T., Takahashi, Y., Tokumoto, U., Yoshimura, T., and Esaki, N. (2002) Cys-328 of IscS and Cys-63 of IscU are the sites of disulfide bridge formation in a covalently bound IscS/IscU complex: implications for the mechanism of iron-sulfur cluster assembly., Proc. Natl. Acad. Sci. U. S. A. 99, 5948-5952.

26. Fukuyama, K., Okada, T., Kakuta, Y., and Takahashi, Y. (2002) Atomic resolution structures of oxidized [4Fe-4S] ferredoxin from Bacillus thermoproteolyticus in two crystal forms: systematic distortion of [4Fe-4S] cluster in the protein., J. Mol. Biol. 315, 1155-1166.

25. Kakuta, Y., Horio, T., Takahashi, Y., and Fukuyama, K. (2001) Crystal structure of Escherichia coli Fdx, an adrenodoxin-type ferredoxin involved in the assembly of iron-sulfur clusters., Biochemistry 40, 11007-11012.

24. Tokumoto, U., and Takahashi, Y. (2001) Genetic analysis of the isc operon in Escherichia coli involved in the biogenesis of cellular iron-sulfur proteins., J. Biochem. 130, 63-71.

23. Ollagnier-de-Choudens, S., Mattioli, T., Takahashi, Y., and Fontecave, M. (2001) Iron-sulfur cluster assembly: characterization of IscA and evidence for a specific and functional complex with ferredoxin., J. Biol. Chem. 276, 22604-22607.

22. Saeki, K., Takahashi, Y., Oh-Oka, H., Umeoka, T., Oda, Y., and Fukuyama, K. (2001) Primary structure and phylogenetic analysis of the coat protein of a Toyama isolate of tobacco necrosis virus., Biosci. Biotechnol. Biochem. 65, 719-724.

21. Oda, Y., Saeki, K., Takahashi, Y., Maeda, T., Naitow, H., Tsukihara, T., and Fukuyama, K. (2000) Crystal structure of tobacco necrosis virus at 2.25 A resolution., J. Mol. Biol. 300, 153-169.

20. Takahashi, Y., and Nakamura, M. (1999) Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli., J. Biochem. 126, 917-926.

19. Nakamura, M., Saeki, K., and Takahashi, Y. (1999) Hyperproduction of recombinant ferredoxins in Escherichia coli by coexpression of the ORF1-ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster., J. Biochem. 126, 10-18.

18. Matsubara, H., Oh-oka, H., Takahashi, Y., and Fujita, Y. (1995) Three iron-sulfur proteins encoded by three ORFs in chloroplasts and cyanobacteria., Photosyn. Res. 46, 107-115.

17. Fujita, Y., Matsumoto, H., Takahashi, Y., and Matsubara, H. (1993) Identification of a nifDK-like gene (ORF467) involved in the biosynthesis of chlorophyll in the cyanobacterium Plectonema boryanum., Plant Cell Physiol. 34, 305-314.

16. Fujita, Y., Takahashi, Y., Chuganji, M., and Matsubara, H. (1992) The nifH-like (frxC) gene is involved in the biosynthesis of chlorophyll in the filamentous cyanobacterium Plectonema boryanum., Plant Cell Physiol. 33, 81-92.

15. Takahashi, Y., Shonai, F., Fujita, Y., Kohchi, T., Ohyama, K., and Matsubara, H. (1991) Structure of co-transcribed gene cluster of ndh1-frxB-ndh6-ndh4L cloned from the filamentous cyanobacterium Plectonema boryanum., Plant Cell Physiol. 32, 969-981.

14. Takahashi, Y., Mitsui, A., and Matsubara, H. (1991) Formation of the Fe-S cluster of ferredoxin in lysed spinach chloroplasts., Plant Physiol. 95, 97-103.

13. Takahashi, Y., Mitsui, A., Fujita, Y., and Matsubara, H. (1991) Roles of ATP and NADPH in formation of the Fe-S cluster of spinach ferredoxin., Plant Physiol. 95, 104-110.

12. Oh-oka, H., Itoh, S., Saeki, K., Takahashi, Y., and Matsubara, H. (1991) FA/FB protein from the spinach photosystem I complex: isolation in a native state and some properties of the iron-sulfur clusters., Plant Cell Physiol. 32, 11-17.

11. Fujita, Y., Takahashi, Y., Shonai, F., Ogura, Y., and Matsubara, H. (1991) Cloning, nucleotide sequence and differential expression of the nifH and nifH-like (frxC) genes from the filamentous nitrogen-fixing cyanobacterium Plectonema boryanum., Plant Cell Physiol. 32, 1093-1106.

10. Oh-oka, H., Takahashi, Y., and Matsubara, H. (1989) Topological consideration of the 9 kDa polypeptide which contains centers A and B, associated with the 14- and 19-kDa polypeptides in the photosystem I complex of spinach., Plant Cell Physiol. 30, 869-875.

9. Fujita, Y., Takahashi, Y., Kohchi, T., Ozeki, H., Ohyama, K., and Matsubara, H. (1989) Identification of a novel nifH-like (frxC) protein in chloroplasts of the liverwort Marchantia polymorpha., Plant molecular biology 13, 551-561.

8. Oh-oka, H., Takahashi, Y., Matsubara, H., and Itoh, S. (1988) EPR studies of a 9 kDa polypeptide with an iron-sulfur cluster(s) isolated from photosystem I complex by n-buthanol extraction., FEBS Lett. 234, 291-294.

7. Oh-oka, H., Takahashi, Y., Kuriyama, K., Saeki, K., and Matsubara, H. (1988) The protein responsible for center A/B in spinach photosystem I: isolation with iron-sulfur cluster(s) and complete sequence analysis., J. Biochem. (Tokyo) 103, 962-968.

6. Oh-oka, H., Takahashi, Y., Wada, K., Matsubara, H., Ohyama, K., and Ozeki, H. (1987) The 8 kDa polypeptide in photosystem I is a probable candidate of an iron-sulfur center protein coded by the chloroplast gene frxA., FEBS Lett. 218, 52-54.

5. Takahashi, Y., Mitsui, A., Hase, T., and Matsubara, H. (1986) Formation of the iron-sulfur cluster of ferredoxin in isolated chloroplasts., Proc. Natl. Acad. Sci. U. S. A. 83, 2434-2437.

4. Minami, Y., Sugimura, Y., Wakabayashi, S., Wada, K., Takahashi, Y., and Matsubara, H. (1985) Isolation, properties, and amino acid sequence of a ferredoxin from a multinuclear, unicellular green alga, Bryopsis maxima., Physiol. Veg. 23, 669-678.

3. Takahashi, Y., Hase, T., Wada, K., and Matsubara, H. (1983) Ferredoxin in developing spinach cotyledons: the presence of two molecular species, Plant Cell Physiol. 24, 189-198.

2. Takahashi, Y., Hase, T., Matsubara, H., Hutber, G. N., and Rogers, L. J. (1982) Amino acid sequence of Chlorogloeopsis fritschii ferredoxin: taxonomic and evolutionary aspects., J. Biochem. (Tokyo) 92, 1363-1368.

1. Takahashi, Y., Hase, T., Wada, K., and Matsubara, H. (1981) The second ferredoxin from spinach leaves., J. Biochem. (Tokyo) 90, 1825-1828.

総説、 Proceedings、著書など

21. Fujishiro, T., Nakamura, R., Kunichika, K., Takahashi, Y. (2022) “Structural diversity of cysteine desulfurases involved in iron-sulfur cluster biosynthesis” Biophys. Physicobiol. 19, e1900001.

20. 藤城貴史、中村亮裕、高橋康弘、(2021) “鉄硫黄クラスター生合成系の硫黄供給酵素群: 共通構造と機能分化・多様性” 生物物理  61: 180-182.

19. Kanazawa, M., Tanaka, N., Matsushima, Y., Takahashi, Y. (2016) “Genetic analysis of suppressor mutations that bypass the essential components of iron-sulfur cluster biosynthesis system.” Genes Genetic Systems, meeting abstract 3E-11. 91(6), 380.

18. Hirabayashi, K., Takahashi, Y., Fukuyama, K., Wada, K. (2012) “Structural basis of the protein-protein interactions among ISC proteins involved in de novo Fe-S clusters biosynthesis.” Acta Crystallogr. A-Foundation and Advances, meeting abstract, MS2-P1. 68, S146.

17. Tanaka, N., Wada K., Fukuyama, K., Takahashi, Y. (2011) “Functional analysis of IscU, a central component of the Fe-S cluster biosynthesis machinery.” Genes Genetic Systems, meeting abstract. 86(6), 407.

16. Hirabayashi, K., Takahashi, Y., Fukuyama, K., Wada, K. (2011) “Crystallographic analyses of the ISC proteins involved in de novo Fe-S cluster biogenesis.” Acta Crystallogr. A-Foundation and Advances, meeting abstract, MS93.P75. 67, C795.

15. 和田啓、福山惠一、高橋康弘、(2010) “ 鉄硫黄クラスターの生合成マシナリーの構造と作動機構” 化学と生物 48:831-838.

14. 高橋康弘、和田啓、福山惠一、(2010) “超分子マシナリーが担う鉄硫黄クラスターの生合成” 生化学 85:139-143.

13. 杉島正一, 萩原義徳, 高橋康弘, 福山惠一、(2006) “フェレドキシン依存性ビリン還元酵素の構造研究”日本結晶学会誌 48:280-289.

12. 南善子、高橋康弘、松原央 (1995) "広く生体反応に関わる鉄硫黄蛋白質" 生物物理、35(2): 7-12

11. 高橋康弘,佐伯和彦,大岡宏造,福山恵一 (1993) "フェレドキシンの進化" 日本生化学会編 新生化学実験法講座16, pp. 153-168

10. 高橋康弘 (1992) "生体膜の調製法 葉緑体" 日本生化学会編 新生化学実験法講座6, pp.420-424

9. J. Stenesh,中村運 他108名 (1992) "分子生物学辞典" 化学同人

8. Fujita, Y., Takahashi, Y., Shonai, F., Matsumoto H., and Matsubara H. (1992) The Light-Independent reduction of protochlorophyllide in the cyanobacterium Plectonema boryanum. In N. Murata ed, Research in Photosynthesis, Vol.III, 67-70

7. Fujita, Y., Takahashi, Y., Oh-oka, H., Saeki, K., Fukuyama, K., and Matsubara, H. (1992) Evolutionary aspects of iron-sulfur proteins in photosynthetic apparatus." In N. Murata ed, Research in Photosynthesis, Vol.III, 491-498

6. Saeki, K., Oh-oka, H., Takahashi, Y., and Matsubara, H. (1991) Ferredoxin and related proteins of Rhodobacter capsulatus and thylakoid membranes." Journal of Inorganic Chemistry 43(2-3):236

5. Oh-oka, H., Takahashi Y., and Matsubara H. (1990) The 9-kDa polypeptide with iron-sulfur center A/B in spinach photosystem I with special reference to its structure and topological consideration in thylakoid membrane. In Bioenergetics: Molecular Biology, Biochemistry, and Pathology (Edited by C. H. Kim and T. Ozawa), pp.261-270, Plenum Press, New York

4. Matsubara, H., Oh-oka, H., Takahashi, Y. and Saeki, K. (1988) A protein carrying Fe-S center A/B in spinach chloroplast photosystem I (PS I)." The FASEB J. 2(4): 768

3. 高橋康弘、松原央 (1986) "鉄−硫黄クラスター合成系の発見" 化学 41(5): 58-59

2. Takahashi, Y., Mitsui, A., Hase, T., and Matsubara, H. (1986) Formation of iron-sulfur iluster of ierredoxin in ihloroplasts. In Iron-Sulfur Protein Research, (eds. H. Matsubara, Y. Katsube and K. Wada), pp 294-301 Japan Sci. Soc. Press. Tokyo/Springer-Verlag, Berlin

1. Takahashi, Y., Hase T., Matsubara H., Hutber G. N. and Rogers L. J. (1981) Amino acid sequence of ferredoxin from the cyanobacterium Chlorogloeopsis fritschii. Biochem. Soc. Trans. 9: 327

主な著書・論文

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