Profile
Nationality : Bangladeshi
Keywords : cell cycle, programmed cell death, FRET, caspase, separase
Research advisor : Professor Miho Suzuki
Why I entered GSC program
Cell and Molecular Biology is an interdisciplinary field of science that deals with the fields of chemistry and biology as it explores to find out life and cellular processes at the molecular level. Cell and Molecular Biology mainly focuses on the determination of cell fate, growth and differentiation of cells, cell-cell contact and movement, or cellular dynamics. Analysis of relationship of cell signaling to cellular growth and death, other cellular dynamics such as transcriptional regulation, mitosis, cellular differentiation and organogenesis, cell-cell contact, might be highly diverse and time dependent. So, it sometimes requires developing specific tool for observation of live cells. As a biologist, I am highly interested to investigate the relationship between programmed cell death and cell cycle. So, I select GSC program to persue my higher study and wish every success of this program.
Research title
Cell based assay for relationship between programmed cell death and cell cycle
Research abstract
Cell proliferation and death must be regulated to maintain tissue homeostasis in multicellular organisms. This regulation may be achieved, in part, by coupling the process of cell cycle progression and programmed cell death by using and controlling a shared set of factors. Deregulation of cell proliferation and/or programmed cell death affects the physiology of an organism. Potentially, this could lead to pathological conditions, including cancer and Alzheimer disease. Apoptosis, most typical programmed cell death, might be adequate to exploit as model system. Though huge number of biological molecules have been established to contribute apoptotic pathways, many biologists have paid attention to a cysteine protease family involved in apoptotic signaling. There are so many such key molecules for cell cycle signaling as well. I have developed molecules sensors, bioprobes to monitor activities for part of key molecules. They are based on Förster resonance energy transfer (FRET); the connected donor and acceptor fluorophore might be changed upon detection of biological activities. I will introduce a green fluorescent protein (GFP) containing a protein digestible sequence near a site complexed with fluorescent dye functions as a separase and caspase due to my objects as key molecules cell lines independently. Then, I will observe separase and caspase activities upon cell cycle and apoptosis inductions by live cell imaging and flow cytometric analysis using bioprobe introduced cancer cell lines. I try to uncover some relationships between programmed cell death and cell cycle through investigate key molecules activities of alive cells.
Graphical abstract
