PSRU_Publications

Publications

Yuzuru Tozawa

Yuhta Nomura

2022

　Original papers

Fu Kuroiwa, Akira Nishino, Yasuko Mandal, Masataka Honzawa, Miki Suenaga-Hiromori, Kakeru Suzuki, Yukie Takani, Yukino Miyagi-Inoue, Haruhiko Yamaguchi, Satoshi Yamashita, Seiji Takahashi, Yuzuru Tozawa. Reconstitution of prenyltransferase activity on nanodiscs by components of the rubber synthesis machinery of the Para rubber tree and guayule. Scientific Reports 12: 3734 (2022) doi: 10.1038/s41598-022-07564-y

2021

　Original papers

Teng Ma, Madoka Sato, Maki Komiya, Kensaku Kanomata, Takaya Watanabe, Xingyao Feng, Ryusuke Miyata, Daisuke Tadaki, Fumihiko Hirose, Yuzuru Tozawa, Ayumi Hirano-Iwata. Lateral voltage as a new input for artificial lipid bilayer systems. Faraday Discussions (2021) https://doi.org/10.1039/D1FD00045D
Natsuki Takamura, Akihiko Yamazaki, Nozomi Sakuma, Sakiko Hirose, Yukie Takani, Satoshi Yamashita, Motonari Sakai, Masahiro Oshima, Makoto Kuroki, Yuzuru Tozawa. Catalytic Promiscuity of Rice 2-Oxoglutarate/Fe(II)–Dependent Dioxygenases Supports Xenobiotic Metabolism. Plant Physiology 187(2):816-828 (2021) doi.org/10.1093/plphys/kiab293

Arif Nurkanto, Ghulam Jeelani, Herbert J Santos, Yulia Rahmawati, Mihoko Mori, Yumi Nakamura, Kana Goto, Yuko Saikawa, Takeshi Annoura, Yuzuru Tozawa, Takaya Sakura, Daniel Ken Inaoka, Kazuro Shiomi, Tomoyoshi Nozaki. Characterization of Plasmodium falciparum pantothenate kinase and identification of its inhibitors from microbial natural products. Frontiers in Cellular and Infection Microbiology 11: 639065 (2021) doi. org/10.3389/fcimb.2021.639065.

2020

　Original papers

Akira Nozawa, Daisuke Ito, Mohamed Ibrahim, Herbert Santos, Takafumi Tsuboi, Yuzuru Tozawa. Characterization of mitochondrial carrier proteins of malaria parasite Plasmodium falciparum based on in vitro translation and reconstitution. Parasitol. Int. 79:102160. (2020) doi.org/10.1016/j.parint.2020.102160.
Kakeru Suzuki, Haruka Inoue, Satoshi Matsuoka, Ryugo Tero, Ayumi Hirano-Iwata, Yuzuru Tozawa. Establishment of a cell-free translation system from rice callus extracts. Biosci. Biotechnol. Biochem. 84:2028-2036 (2020) doi.org/10.1080/09168451.2020.1779024.
Daniel Moog, Akira Nozawa, Yuzuru Tozawa, Ryoma Kamikawa. Substrate specificity of plastid phosphate transporters in a non-photosynthetic diatom and its implication in evolution of red alga-derived complex plastids. Sci. Rep. 10:1167 (2020) doi.org/10.1038/s41598-020-58082-8

　Review

Maki Komiya, Miki Kato, Daisuke Tadaki, Teng Ma, Hideaki Yamamoto, Ryugo Tero, Yuzuru Tozawa, Michio Niwano, Ayumi Hirano-Iwata. Advances in artificial cell membrane systems as a platform for reconstituting ion channels. Chemical Record 20:1-14 (2020)

2019

　Original papers

Maeda H, Murata K, Sakuma N, Takei S, Yamazaki A, Karim MR, Kawata M, Hirose S, Kawagishi-K M, Taniguchi Y, Suzuki S, Sekino K, Ohshima M, Kato H, Yoshida H, Tozawa Y. A rice gene that confers broad-spectrum resistance to beta-triketone herbicides. Science 365: 393-396. doi: 10.1126/science.aax0379. (2019)

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Santos H, Imai K, Makiuchi T, Tomii K, Horton P, Nozawa A, Okada K, Tozawa Y, Nozaki T. Novel lineage-specific transmembrane β-barrel proteins in the endoplasmic reticulum of Entamoeba histolytica. FEBS J. doi: 10.1111/febs.14870 (2019)
Seki T, Furumi T, Hashimoto M, Hara H, Matsuoka S. Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid. Genes Genet. Syst. 94:71-80 (2019)

2018

　Original papers

Imamura S, Nomura Y, Takemura T, Pancha I, Taki K, Toguchi K, Tozawa Y, Tanaka K. The checkpoint kinase TOR (target of rapamycin) regulates expression of a nuclear-encoded chloroplast RelA-SpoT homolog (RSH) and modulates chloroplast ribosomal RNA synthesis in a unicellular red alga. Plant J. i2018 Feb 14. doi: 10.1111/tpj.13859. (2018)
Saito S, Hamamoto S, Moriya K, Matsuura A, Sato Y, Muto J, Noguchi H, Yamauchi S, Tozawa Y, Ueda M, Hashimoto K, Köster P, Dong Q, Held K, Kudla J, Utsumi T, Uozumi N. N-myristoylation and S-acylation are common modifications of Ca2+ regulated Arabidopsis kinases and are required for activation of the SLAC1 anion channel. New Phytol. Mar 2. doi: 10.1111/nph.15053. (2018)

2017

　Original papers

Tadaki D, Yamaura D, Araki S, Yoshida M, Arata K, Ohori T, Ishibashi K-I, Kato M, Ma T, Miyata R, Tozawa Y, Yamamoto H, Niwano M, Hirano-Iwata A. Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels. Sci. Rep. 7: 17736 (2017)
Beljantseva J, Kudrin P, Jimmy S, Ehn M, Pohl R, Varik V, Tozawa Y, Shingler V, Tenson T, Rejman D, Hauryliuk V. Molecular mutagenesis of ppGpp: turning a RelA activator into an inhibitor. Sci. Rep. 7: 41839 (2017)
Seki T, Matsumoto K, Matsuoka S, and Hara H. Activation without Proteolysis of Anti-σ Factor RsiV of the Extracytoplasmic Function σ Factor σ^V in a Glucolipid-Deficient Mutant of Bacillus subtilis. Adv. Microbiol. 7:315-327. (2017)
Ishikawa K, Matsuoka S, Hara H, Matsumoto K. Septal membrane localization by COOH-terminal amphipathic α-helices of MinD in Bacillus subtilis mutant cells lacking MinJ or DivIVA. Genes Genet. Syst. 92:81-98 (2017)
Sato T, Takano A, Hori N, Izawa T, Eda T, Sato K, Umekawa M, Miyagawa, Matsumoto K, Muramatsu-Fujishiro A, Matsumoto K, Matsuoka S, and Hara H. Role of the inner-membrane histidine kinase RcsC and outer-membrane lipoprotein RcsF in the activation of the Rcs phosphorelay signal transduction system in Escherichia coli. Microbiology 163:1071-1080 (2017)

　Review, Proceedings

Matsuoka S. Biological functions of glucolipids in Bacillus subtilis ugtP. Genes Genet. Syst. 92:217-221 (2017)

2016

　Original papers

Yamashita S, Yamaguchi H, Waki T, Aoki Y, Mizuno M, Yanbe F, Ishii T, Funaki A, Tozawa Y, Miyagi-Inoue Y, Fushihara K, Nakayama T, Takahashi S. Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliensis. Elife 5: e19022 (2016)
Andresen L, Varik V, Tozawa Y, Jimmy S, Lindberg S, Tenson T, Hauryliuk V. Auxotrophy-based High Throughput Screening assay for the identification of Bacillus subtilis stringent response inhibitors. Sci. Rep. 6: 35824 (2016)
Matsuoka S, Seki T, Matsumoto K, and Hara H. Suppression of abnormal morphology and extracytoplasmic function sigma activity in Bacillus subtilis ugtP mutant cells by expression of heterologous glucolipid synthases from Acholeplasma laidlawii. Biosci. Biotechnol. Biochem. 80:2325-2333. (2016)
Kusaka J, Shuto S, Imai Y, Ishikawa K, Saito T, Natori K, Matsuoka S, Hara H, and Matsumoto K. Septal localization by membrane targeting sequences and a conserved sequence essential for activity at the COOH-terminus of Bacillus subtilis cardiolipin synthase. Res. Microbiol. 167:202-214. (2016)

2015

　Original papers

Mi-ichi F, Nozawa A, Yoshida H, Tozawa Y*, Nozaki T*. Evidence that Entamoeba histolytica mitochondrial carrier family links mitosomal and cytosolic pathways through exchange of PAPS and ATP. Eukaryot. Cell 14:1144-1150 (2015)
Watanabe S, Hiraoka Y, Endo S, Tanimoto Y, Tozawa Y, Watanabe Y. An Enzymatic method to estimate the content of L-hydroxyproline. J. Biotechnol. 199:9-16 (2015)
Santos HJ, Imai K, Makiuchi T, Tomii K, Horton P, Nozawa A, Ibrahim M, Tozawa Y, Nozaki T. A Novel β-Barrel Mitosomal Outer Membrane Protein in Entamoeba. Sci. Rep. 5:8545 (2015)
Seki T, Mineshima R, Hashimoto M, Matsumoto K, Hara H, and Matsuoka S. Repression of the activities of extracytoplasmic function σ factors, sigma M and sigma V, of Bacillus subtilis by glucolipids in Escherichia coli cells. Genes Genet. Syst. 90:109-114. (2015)

2014

　Original papers

Watanabe S, Tozawa Y, Watanabe Y. Ornithine cyclodeaminase/μ-crystallin homolog from hyperthermophilic archaeon Thermococcus litoralis functions as a novel Δ1-pyrroline-2-carboxylate reductase involved in putative trans-3-hydroxy-L-proline metabolism. FEBS Open Bio 4:617-626 (2014)
Nomura Y, Izumi A, Fukunaga Y, Kusumi K, Iba K, Watanabe S, Nakahira Y, Weber AMP, Nozawa A, Tozawa Y. Diversity in Guanosine 3',5'-Bisdiphosphate (ppGpp) Sensitivity Among Guanylate Kinases of Bacteria and Plants. J. Biol. Chem. 289:15631-15641 (2014)
Nomura Y, Nozawa A, Tozawa Y. Biochemical analyses of ppGpp effect on adenylosuccinate synthetases, key enzymes in purine biosynthesis in rice. Biosci. Biotechnol. Biochem. 78:1022-1025 (2014)
Nozawa A, Tozawa Y. Incorporation of adenosine nucleotide transporter, ANT1, into proteoliposomes facilitates ATP translocation and activation of encapsulated luciferase. J. Biosci. Bioeng. S1389-1723(14)00035-8. (2014)
Watanabe S, Tanimoto Y, Yamauchi S, Tozawa Y, Sawayama S, Watanabe Y. Identification and Characterization of trans-3-Hydroxy-L-proline Dehydratase and <DELTA>¹-Pyrroline-2-carboxylate Reductase Involved in trans-3-Hydroxy-L-proline Metabolism of Bacteria. FEBS Open Bio 4:240-250 (2014)

　Review, Proceedings

Nozawa A, Tozawa Y. Modifications of wheat germ cell-free system for functional proteomics of plant membrane proteins. Methods in Molecular Biology. Springer Science+Business Media, LLC, New York, NY. 1072: 259-272 (2014)

2013

　Original papers

Nakahira Y, Ogawa A, Asano H, Oyama T, Tozawa Y. Theophylline-dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC7942. Plant Cell Physiol. 54:1724-1735 (2013)
Nakahira Y, Ishikawa K, Tanaka k, Tozawa Y, Shiina T. Overproduction of hyperthermostable β-1,4-endoglucanase from the archaeon Pyrococcus horikoshii by tobacco chloroplast engineering. Biosci. Biotechnol. Biochem. 77: 2140-2143 (2013)
Kore-eda S, Nozawa A, Okada Y, Nishiyama Y, Tozawa Y. Characterization of phosphoenolpyruvate/phosphate translocator from iceplant. Biosci. Biotechnol. Biochem. 77:1511-1516 (2013)
Kubota S, Shirai O, Hibi T, Tozawa Y, Kano K. Effect of counter ions on the transport current across membranes containing KAT1 potassium channel. Analytical Sciences 29:161-164 (2013)

　Review, Proceedings

Bernhard F, Tozawa Y. Cell-Free Expression - Making a Mark. Curr. Opin. Struct. Biol. 23: 374-380 (2013)

2012

　Original papers

Watanabe S, Morimoto D, Fukumori F, Shinomiya H, Nishiwaki H, Kawano-Kawada M, Sasaki Y, Tozawa Y, Watanabe Y. Identification and characterization of D-Hydroxyproline dehydrogenase and Δ1-Pyrroline-4-Hydroxy-2-Carboxylate Deaminase involved in novel L-Hydroxyproline metabolism of bacteria: METABOLIC CONVERGENT EVOLUTION. J. Biol. Chem. 283:32674-32688 (2012)
Kato H, Watanabe S, Nimura-Matsune K, Chibazakura T, Tozawa Y, Yoshikawa H. Exploration of the possible partnership amoung orphan two-component system proteins in cyanobacterium Synechococcus elongates PCC 7942. Biosci. Biotechnol. Biochem. 76:1484-1491(2012)
Yagi Y, Ishizaki Y, Nakahira Y, Tozawa Y, Shiina T. Non-prokaryotic nucleoid protein pTAC3 is essential for transcription by a cyanobacteria-derived RNA polymerase in chloroplasts. Proc. Natl. Acad. Sci. USA 109:7541-7546 (2012)
Tagami K, Nanamiya H, Kazo Y, Maehashi M, Suzuki S, Namba E, Tozawa Y, Morimoto T, Ogasawara N, Kageyama Y, Ara K, Ozaki K, Yoshida M, Kuroiwa T, Ohashi Y, Kawamura F. Expression of small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome. MicrobiologyOpen 1:115-134 (2012)
Nomura Y, Takabayashi T, Kuroda H, Yukawa Y, Sattasuk K, Akita M, Nozawa A, Tozawa Y. ppGpp inhibits peptide elongation cycle of chloroplast translation system in vitro. Plant Mol. Biol. 78:185-196 (2012)

　Review, Proceedings

Tozawa Y. Cell-free protein synthesis of membrane-integrated proteins for functional analysis. (2011) Frontiers in Agriculture Proteome Research, Contribution of Proteomics Technology in Agricultural Sciences, Proceedings of the 3rd International Symposium on “Frontiers in Agriculture Proteome Research”, Eds., Mock HP, Wang ZY, and Komatsu S. 16-23 (2012)

2011

　Original papers

Sattasuk K, Nozawa A, Tozawa Y, Kakinuma Y, Akita M. In vitro protein import of a putative amino acid transporter from Arabidopsis thaliana into chloroplasts and its suborganellar localization. Biosci. Biotechnol. Biochem. 75:2200-2006 (2011)
Nozawa A, Fujimoto R, Matsuoka H, Tsuboi T, Tozawa Y. Cell-free protein synthesis, reconstitution and characterization of mitochondrial dicarboxylate-tricarboxylate carrier of Plasmodium falciparum. Biochem. Biophys. Res. Commun. 414:612-617 (2011)
Tozawa Y, Nomura Y. Signaling by the global regulatory molecule ppGpp in bacteria and chloroplasts of land plants. Plant Biol. 13:609-709 (2011)
Narimatsu Y, Kubota T, Furukawa S, Shimojima M, Iwasaki H, Tozawa Y, Tachibana K, Narimatsu H. Co-translational function of Cocmc, core 1 synthase specific molecular chaperine, revealed by a cell-free translation system. FEBS Lett. 585:1276-1280 (2011)
Ito K, Nozaki M, Ohta T, Miura C, Tozawa Y, Miura T. Differences of two polychaete species reflected in enzyme activities. Marine Biol. 158:1211-1221 (2011)
Ohta T, Ueda Y, Ito K, Celino FT, Miura C, Yamashita H, Miura T, Tozawa Y. Anti-viral effects of interferon administration on Sevenband grouper, Epinephelus septemfasciatus. Fish Shellfish Immunol. 30:1064-1071 (2011)
Celino FT, Yamaguchi S, Miura C, Ohta T, Tozawa Y, Iwai T, Miura T. Tolerance of spermatogonia to oxidatve stress is due to high levels of Zn and Cu/Zn superoxide dismutase. PLoS ONE 6:e16938 (2011)

2010

　Original papers

Genji T, Nozawa A, and Tozawa Y. Efficient production and purification of functional bacteriorhodopsin with a wheat-germ cell-free system and a combination of Fos-choline and CHAPS detergents. Biochem. Biophys. Res. Commun. 400:638-642 (2010)
Chadani Y, Ono K, Ozawa S-I, Takahashi Y, Takai K, Nanamiya H, Tozawa Y, Kutsukake K, and Abo T. Ribosome rescue by Escherichia coli AffA (YhdL) in the absence of trans-translation system. Mol. Microbiol. 78:796-808 (2010)
Yamauchi S, Fusada N, Hayashi H, Utsumi T, Uozumi N, Endo Y, and Tozawa Y. The concensus motif for N-myristoylation of plant proteins in a wheat germ cell-free translation system. FEBS J. 277:3596-3607 (2010)
Nishiyama Y, Yun CS, Masuda F, Sasaki T, Saito K, and Tozawa Y. Expression of bacterial tyrosine ammonia-lyase creates a novel p-coumaric acid pathway in the biosynthesis of phenylpropanoids in Arabidopsis thaliana. Planta 232 (1):201-218 (2010)
Ohta T, Matsuoka H, Nomura Y, and Tozawa Y. Control of translational initiation in the wheat-embryo cell-free protein expression system for producing homogenous products. Protein Expr. Purif. 73:15-22 (2010)

　Review, Proceedings

Nozawa A, Nanamiya H, and Tozawa Y. Production of membrane proteins through the wheat-germ cell-free technology. Methods in Molecular Biology. Humana Press Inc., Totowa, NJ. 607:213-218 (2010)
Kanno T, and Tozawa Y. Protein engineering accelerated by cell-free technology. Methods in Molecular Biology. Humana Press Inc., Totowa, NJ. 607:85-99 (2010)

2009

　Original papers

Natori Y, Tagami K, Murakami K, Yoshida S, Tanigawa O, Masuda K, Wada T, Suzuki S, Nanamiya H, Tozawa Y, and Kawamura F. Transcription activity of individual rrn operons in Bacillus subtilis mutants deficient of (p)ppGpp synthetase genes, relA, yjbM, and ywaC. J. Bacteriol.191 (14):4555-4561 (2009)
Nozaki M, Miura C, Tozawa Y, and Miura T. The contribution of endogenous-cellulose digestion in the gut of earthworm (Pheretima hilgendorfi:Megascolecidae). Soil Biol. Biochem. 41:762-769 (2009)
Yun CS, Hasegawa H, Terakawa T, and Tozawa Y. Novel bacterial N-acetyltransferase gene for herbicide detoxification in land plants and selectable marker in plant transformation. Biosci. Biotechnol. Biochem. 73(5):1000-1006 (2009)

　Review, Proceedings

2008

　Original papers

Masuda S, Tozawa Y, and Ohta H. Possible targets of "magic spots" in plant signaling. Plant Signaling & Behavior 3 (11): 1021-1023 (2008)
Yamada T, Matsuda F, Kasai K, Fukuoka S, Kitamura K, Tozawa Y, Miyagawa H, and Wakasa K. Mutation of an enzyme that functions in phenylalanine biosynthesis results in accumulation of phenylalanine and tryptophan in rice. Plant Cell 20 (5):1316-1329 (2008)
Yun CS, Yamamoto T, Nozawa A, Tozawa Y. Expression of parsley flavone synthase I establishes the flavone biosynthetic pathway in Arabidopsis thaliana. Biosci. Biotechnol. Biochem.72 (4): 968-973 (2008)
Masuda S, Mizusawa K, Narisawa T, Tozawa Y, Ohta H, and Takamiya KI. The Bacterial Stringent Response, Conserved in Chloroplasts, Controls Plant Fertilization. Plant Cell Physiol. 49 (2):135-141 (2008)
Nanamiya H, Kasai K, Nozawa A, Yun C-S, Narisawa T, Murakami K, Natori Y, Kawamura F, and Tozawa Y. Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis. Mol. Microbiol. 67 (2): 291-304 (2008)

　Review, Proceedings

Nozaki M, Miura C, Tozawa Y, and Miura T. The role of the earthworm, Pheretima (Metaphire) holgendorfi, in terrestrial ecosystem nutrient cycling. Interdisciplinary Studies on Environmental Chemistry–Biological Responses to Chemical Pollutants, Eds., Murakami Y, Nakayama N, Kitamura S-I, Iwata H, and Tanabe S. 275-279 (2008)
Tagami K, Wada T, Maehashi M, Hirohata Y, Yoshida M, Kuroiwa H, Kuroiwa T, Nanamiya H, Tozawa Y, Liu S, Kageyama Y, Ara K, Ozaki K, Kawamura F. Functional analysis of a novel ppGpp synthetase, YwaC, and regulatory mechanism for the dimerization of ribosome in Bacillus subtilis. Genes & Genetics Systems 83:513 (2008)
Tagami K, Wada T, Yoshida M, Kuroiwa H, Kuroiwa T, Nanamiya H, Tozawa Y, Kawamura F. Functional analysis of a novel ppGpp synthetase, YwaC, in Bacillus subtilis. Genes & Genetics Systems 83:513 (2008)

2007

　Original papers

Nozawa A, Nanamiya H, Mityata T, Linka N, Endo Y, Weber APM, and Tozawa Y. A cell-free translation and proteoliposome-reconstitution system for functional analysis of plant solute transporters. Plant Cell Physiol. 48 (12): 1815-1820 (2007)
Tozawa Y, Nozawa A, Kanno T, Narisawa T, Masuda S, Kasai K, Nanamiya, H. Calcium-activated (p)ppGpp synthetase in chloroplasts of land plants. J. Biol. Chem. 282 (49): 35536-35545 (2007)
Tozawa Y, Teraishi M, Sasaki T, Sonoike K, Nishiyama Y, Itaya M, Miyao A, and Hirochika H. The plastid sigma factor SIG1 maintains photosystem I activity via regulated expression of the psaA operon in rice chloroplasts. Plant J. 52: 124-132 (2007)
Kojima K, Oshita M, Nanjo Y, Kasai K, Tozawa Y, Hayashi H, and Nishiyama Y. Oxidation of elongation factor G inhibits the synthesis of the D1 protein of photosystem II. Mol. Microbiol. 64: 936-947 (2007)
Kubota Y, Miyao A, Hirochika H, Tozawa Y, Yasuda H, Tsunoyama Y, Niwa Y, Imamura S, Shirai M, and Asayama M. Two novel nuclear genes, OsSIG5 and OsSIG6, encoding potential plastid sigma factors of RNA polymerase in rice: tissue-specific and light-responsive gene expression. Plant Cell Physiol.48: 186-192 (2007)
Kanno T, Kitano M, Kato R, Ohmori A, Endo Y, and Tozawa Y. Sequence specificity and efficiency of protein N-terminal methionine elimination in wheat-embryo cell-free system. Protein Expr. Purif. 52: 59-65 (2007)
Kuroita T, Kanno T, Kawai A, Kawakami B, Oka M, Endo Y, and Tozawa Y. Functional similarities of a thermostable protein-disulfide oxidoreductase identified in the archaeon Pyrococcus horikoshii to bacterial DsbA enzymes. Extremophiles 11: 85-94 (2007)

　Proceedings

Kasai K, Obayashi Y, Yamada T, Kanno T, Wakasa K, Tozawa Y. Functional analysis of arogenate dehydratase of rice by means of wheat-embryo cell-free protein expression system. Plant Cell Physiol.48:S219 (2007)
Tozawa Y, Teraishi M, Sasaki T, Sonoike K. Functional roles of plastid SIG1 for gene expression of photosystem I components. Plant Cell Physiol.48:S35 (2007)

2006

　Original papers

Wakasa K, Hasegawa H, Nemoto H, Matusda F, Miyazawa H, Tozawa Y, Morino K, Komatsu A, Yamada T, Terakawa T, Miyagawa H. High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile. Journal of Experimental Botany 57: 3069-3078 (2006)
Ozaki Y, Higuchi M, Miura C, Yamaguchi S, Tozawa Y, and Miura T. Roles of 11b-hydroxysteroid dehydrogenase in fish spermatogenesis. Endocrinology 147: 5139-5146(2006)
Makino SI, Sawasaki T, Tozawa Y, Endo Y, and Takai K. Covalent circularization of exogenous RNA during incubation with a wheat embryo cell extract. Biochem. Biophys. Res. Commun. 347: 1080-1087 (2006)
Hirano N, Sawasaki T, Tozawa Y, Endo Y, and Takai K. Tolerance for random recombination of domains in prokaryotic and eukaryotic translation systems: limited inter-domain misfolding in a eukaryotic translation system. Proteins 64: 343-354 (2006)

　Proceedings

Murakami K, Natori Y, Sato M, Nanamiya H, Kawamura F, Tozawa Y. Function analysis of relA and it’s paralogous genes, yjbM and ywaC, in Bacillus subtilis. Genes & Genetics Systems 81:442 (2006)

2005

　Original papers

Kanno T, Komatsu A, Kasai K, Dubouzet JG, Sakurai M, Ikejiri-K Y, Wakasa K, and Tozawa Y. Structure-based in vitro engineering of the anthranilate synthase, a metabolic key enzyme in the plant Trp biosynthetic pathway. Plant Physiol. 138: 2260-2268 (2005)
Kasai K, Kanno T, Akita M, Ikejiri-Kanno Y, Wakasa K, and Tozawa Y. Identification of three shikimate kinase genes in rice: characterization of their differential expression during panicle development and of the enzymatic activities of the encoded proteins. Planta 222:438-447 (2005)

　Review, Proceedings

Sawasaki T, Gouda MD, Kawasaki T, Tsuboi T, Tozawa Y, Takai K, and Endo Y. The Wheat Germ Cell-Free Expression System. Methods in Molecular Biology. Humana Press Inc., Totowa, NJ. 310:131-144 (2005)

2004

　Original papers

Kasai K, Kanno T, Wakasa K, Endo Y, and Tozawa Y. Guanosine tetra- and pentaphosphate synthetase activity in chloroplasts of a higher plant: association with 70S ribosomes and inhibition by tetracycline. Nucleic Acids Res. 32: (19) 5732-5741 (2004)
Kusumi K, Yara A, Mitsui N, Tozawa Y, and Iba K. Characterization of a rice nuclear-encoded plastid RNA polymerase gene OsRpoTp. Plant Cell Physiol. 45: (9) 1194-1201 (2004)
Yamada T, Tozawa Y, Hasegawa H, Terakawa T, Ohkawa Y, and Wakasa K. Use of a feedback-insensitive a subunit of anthranilate synthase as a selectable marker for transformation of rice and potato. Molecular Breeding 14: 363-373 (2004)
Sugimoto H, Kusumi K, Tozawa Y, Yazaki J, Kishimoto N, Kikuchi S, and Iba K. The virescent-2 Mutation Inhibits Translation of Plastid Transcripts for the Plastid Genetic System at an early stage of chloroplast differentiation. Plant Cell Physiol. 45: (8)985-996 (2004)
Tanaka N, Fujita M, Handa H, Muryama S, Umemura M, Kawamura Y, Mitsui T, Mikami S, Tozawa Y, Yoshinaga T, and Komatsu S. Proteomics of the rice cell: systematic identification of the protein population in subcellular compartments. Mol. Genet. Genomics 271: (5) 566-576 (2004)
Kanno T, Kasai K, Ikejiri-Kanno Y, Wakasa K, and Tozawa Y. In vitro reconstitution of rice anthranilate synthase: distinct functional properties of the a subunits OASA1 and OASA2. Plant Mol. Biol. 54: (1) 11-23 (2004)
Kasai K, K-Kobayashi M, Teraishi M, Ito Y, Ochi K, Wakasa K, and Tozawa Y. Differential Expression of Three Plastidial Sigma Factors, OsSIG1, OsSIG2A and OsSIG2B, during leaf development in rice. Biosci Biotechnol Biochem. 68: (4) 973-977 (2004)

　Review, Proceedings

Ochi K, Okamoto S, Tozawa Y, Inaoka T, Hosaka T, Xu J, and Kurosawa K. Ribosome Engineering and Secondary Metabolite Production. Advances in Applied Microbiology. Academic Press, Review 56: 155- 184 (2004)
Sugimoto H, Kusumi K, Yoshimura A, Kikuchi S, Tozawa Y, Iba K. Functional analysis of rice virescent-2 gene essential for chloroplast development. Plant Cell Physiol. 45:S208 (2004)

2003

　Original papers

Ohashi Y, Inaoka T, Kasai K, Ito Y, Okamoto S, Satsu H, Tozawa Y, Kawamura F, and Ochi K. Expression Profiling of Translation-associated Genes in Sporulation Bacillus subtilis and Consequence of Sporulation by Gene Inactivation. Biosci Biotechnol Biochem. 67: (10) 2245-2253 (2003)

　Review, Proceedings

Tozawa Y, Sawasaki T, and Endo Y. Cell-free Protein Synthesis by Wheat Germ Extracts. 日本結晶学会誌総説, 45:3-8 (2003)
Kusumi K, Kouno T, Sugimoto H, Tozawa Y, Yoshimura A, Kikuchi S, Iba K. Expression analysis of chloroplast proteins in virescent mutants (v(1), v(3)) of rice. Plant Cell Physiol. 44:S57 (2003)

~2002

　Original papers

Ito Y, Ozawa A, Sawasaki T, Endo Y, Ochi K, and Tozawa Y. OsRALyase1, a Putative F-Box Protein Identified in Rice Oryza sativa, Possesses Enzyme Activity Identical to That of Wheat RALyase. Biosci Biotechnol Biochem. 66: 2725-2729 (2002)
Kasai K, Usami S, Yamada T, Endo Y, Ochi K, and Tozawa Y. A RelA-SpoT homolog (Cr-RSH) identified in Chlamydomonas reinhardtii generates stringent factor in vivo and localizes to chloroplasts in vitro. Nucleic Acids Res. 30: 4985-4992 (2002)
Xu J, Tozawa Y, Lai C, Hyashi H, and Ochi K. Rifampicin resistant mutation in the rpoB gene confers ppGpp-independent antibiotic production to Streptomyces coelicolor A3(2). Mol. Gen. Genet. 268: 179-189 (2002)
Lai C, Xu J, Tozawa Y, Okamoto-H Y, Yao X, and Ochi K. Genetic and physiological characterization of rpoB mutations which activate antibiotic production in Streptomyces lividans. Microbiology 148: 3365-3373 (2002)
Tozawa Y, Hasegawa H, Terakawa T, and Wakasa K. Characterization of rice anthranilate synthase alfa subunit genes OSASA1 and OSASA2: tryptophan accumulation in transgenic rice expressing a feedback-insensitive mutant of OASA1. Plant Physiol. 126: 1493-1506 (2001)
Urushibara S, Tozawa Y, Kawagishi-Kobayashi M, and Wakasa K. Efficient transformation of rice suspension-cultured cells mediated by Agrobacterium tumefaciens. Breeding Science 51: 33-38 (2001)
Asada A, Zhao Y, Komano H, Kuwata T, Mukai M, Fujita K, Tozawa Y, Iseki R, Tian H, Sato K, Motegi K, Suzuki R, Yokoyama M, and Iwata M. The calcium-independent protein kinase C participates in an early process of CD3/CD28-mediated induction of thymocyte apoptosis. Immunology 101: 309-315 (2000)
Tsunoyama Y, Tozawa Y, and Wakasa K. Coexistence of the plastidial sigma factor SigA and bacterial RNA polymerase activity in partially purified chloroplast soluble protein fraction. Rice Genet. Newslett. 15:176-178 (1999)
Tozawa Y, Tanaka K, Takahashi H, and Wakasa K. Nuclear encoding of a plastid sigma factor in rice and its tissue- and light-dependent expression. Nucleic Acids Res. 26:415-419 (1998)
Tanaka K, Tozawa Y, Mochizuki N, Shinozaki K, Nagatani A, Wakasa K, and Takahashi H. Characterization of three cDNA species encoding plastid RNA polymerase sigma factors in Arabidopsis thaliana: evidence for the sigma factor heterogeneity in higher plant plastids. FEBS Lett. 413:309-313 (1997)
Iwata M, Kuwata T, Mukai M, Tozawa Y, and Yokoyama Y. Differential induction of helper and killer T cells from isolated CD4+CD8+ thymocytes in suspension culture. Eur. J. Immunol. 26:2081-2086 (1996)
Ohoka Y, Kuwata T, Tozawa Y, Zhao Y, Mukai M, Motegi Y, Suzuki R, Yokoyama M, and Iwata M. In vitro differentiation and commitment of CD4+ CD8+ thymocytes to the CD4 lineage, without TCR engagement. Int. Immunol. 8:297-306 (1996)
Sato TN, Tozawa Y, Deutsch U, Wolburg-Buchholz K, Fujiwara Y, Gendron-Maguire M, Gridley T, Wolburg H, Risau W, and Qin Y. Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation. Nature 376: 70-74 (1995)
Zhao Y, Tozawa Y, Iseki R, Mukai M, and Iwata M. Calcineurin activation protects T cells from glucocorticoid-induced apoptosis. J. Immunol. 154:6346-6354 (1995)
Iwata M, Iseki R, Sato K, Tozawa Y, and Ohoka Y. Involvement of protein kinase C-epsilon in glucocorticoid-induced apoptosis in thymocytes. Int. Immunol. 6:431-438 (1994)
Tozawa Y, Yoshikawa H, Kawamura F, Itaya M, and Takahashi H. Isolation and characterization of the groES and groEL genes of Bacillus subtilis Marburg. Biosci. Biotechnol. Biochem. 56:1995-2002 (1992)
Yamazaki S, Kobayashi N, Okubo A, Sato T, Tozawa Y, Yoshimura E, Kajiwara H, Hirano H, and Toda S. Charactreization of dimethylsulfoxide reductase, a novel molybdo-enzyme from photosynthetic bacteria, Rhodobacter spheroids f.s. denitrificans. Analytical Sciences 7:821-824 (1991)

　Review, Proceedings

Komatsu A, Yoshikawa A, Hasegawa H, Kawagishi-Kobayashi M, Nishizawa Y, Sugimoto K, Tozawa Y, Wakasa K. Analysis of rice transformants with the mutated anthranilate synthase gene driven by five different promoters fro the development of selection marker. Plant Cell Physiol. 43:S121 (2002)
Tozawa Y, Tanaka K, Wakasa K. Molecular cloning of the gene encoding putative chloroplast RNA polymerase sigma subunit from rice. Plant Physiol. 114:1276 (1997)
Crouch RJ, Tozawa Y, and Cerritelli SM. Ribonuclease H of Saccharomyces pombe and Crithithidia fasciculate share a common moth in their non-RNase-H domain that binds double-stranded-RNA in a magnesium-dependent manner. Protein Engineering 7:1151-1152 (1994)
Tozawa Y, and Sato TN. Roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation. EXPERIMENTAL MEDICINE (実験医学) 14:88-91 (1996)