PSRU_Publications

Publications

Yuzuru Tozawa

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ResearchGate

2024

　Original papers

Riku Nakamura, Yuji Takahashi, Shogo Tachibana, Arisa Terada, Kakeru Suzuki, Kumika Kondo, Yuzuru Tozawa, Yukako Hihara. Partner-switching components PmgA and Ssr1600 regulate high-light acclimation in Synechocystis sp. PCC 6803. Plant Physiology kiae323 (2024)

　Review, Interpretive article

黒岩風，戸澤譲. 天然ゴム酵素複合体の再構成. アグリバイオ 8(6): 475-479 (2024)

2023

　Original papers

Fu Kuroiwa, Hiraku Suda, Maho Yabuki, Kimie Atsuzawa, Haruhiko Yamaguchi, Masatsugu Toyota, Yasuko Kaneko, Satoshi Yamashita, Seiji Takahashi, Yuzuru Tozawa. Cell-free translation system with artificial lipid-monolayer particles as a unique tool for characterizing lipid-monolayer binding proteins. Bioscience, Biotechnology, and Biochemistry 88:555-560 (2023)
Melvin Wei Shern Goh, Yuzuru Tozawa, Ryugo Tero. Assembly of Cell-Free Synthesized Ion Channel Molecules in Artificial Lipid Bilayer Observed by Atomic Force Microscopy. Membranes 13(11):854 (2023)
Madoka Sato, Masanori Hariyama, Maki Komiya, Kae Suzuki, Yuzuru Tozawa, Hideaki Yamamoto, Ayumi Hirano-Iwata. Biophysical Journal 122(19): 3959-3975 (2023)

2022

　Original papers

Fu Kuroiwa, Akira Nishino, Yasuko Mandal, Masataka Honzawa, Miki Suenaga-Hiromori, Kakeru Suzuki, Yukie Takani, Yukino Miyagi-Inoue, Haruhiko Yamaguchi, Satoshi Yamashita, Seiji Takahashi, Yuzuru Tozawa. Reconstitution of prenyltransferase activity on nanodiscs by components of the rubber synthesis machinery of the Para rubber tree and guayule. Scientific Reports 12(1):3734 (2022) doi.org/10.1038/s41598-022-07564-y

2021

　Original papers

Teng Ma, Madoka Sato, Maki Komiya, Kensaku Kanomata, Takaya Watanabe, Xingyao Feng, Ryusuke Miyata, Daisuke Tadaki, Fumihiko Hirose, Yuzuru Tozawa, Ayumi Hirano-Iwata. Lateral voltage as a new input for artificial lipid bilayer systems. Faraday Discussions (2021) https://doi.org/10.1039/D1FD00045D

Natsuki Takamura, Akihiko Yamazaki, Nozomi Sakuma, Sakiko Hirose, Yukie Takani, Satoshi Yamashita, Motonari Sakai, Masahiro Oshima, Makoto Kuroki, Yuzuru Tozawa. Catalytic Promiscuity of Rice 2-Oxoglutarate/Fe(II)–Dependent Dioxygenases Supports Xenobiotic Metabolism. Plant Physiology 187(2):816-828 (2021) doi.org/10.1093/plphys/kiab293

Arif Nurkanto, Ghulam Jeelani, Herbert J Santos, Yulia Rahmawati, Mihoko Mori, Yumi Nakamura, Kana Goto, Yuko Saikawa, Takeshi Annoura, Yuzuru Tozawa, Takaya Sakura, Daniel Ken Inaoka, Kazuro Shiomi, Tomoyoshi Nozaki. Characterization of Plasmodium falciparum pantothenate kinase and identification of its inhibitors from microbial natural products. Frontiers in Cellular and Infection Microbiology 11: 639065 (2021) doi. org/10.3389/fcimb.2021.639065.

　Review, Interpretive article

鈴木香絵, 熊地重文, 戸澤譲, 根本直人. 膜タンパク質を標的とした高速人工ペプチドリガンドスクリーニングシステム. 細胞 53(5): 317-321 (2021)

2020

　Original papers

Akira Nozawa, Daisuke Ito, Mohamed Ibrahim, Herbert Santos, Takafumi Tsuboi, Yuzuru Tozawa. Characterization of mitochondrial carrier proteins of malaria parasite Plasmodium falciparum based on in vitro translation and reconstitution. Parasitology International 79:102160. (2020) doi.org/10.1016/j.parint.2020.102160.
Kakeru Suzuki, Haruka Inoue, Satoshi Matsuoka, Ryugo Tero, Ayumi Hirano-Iwata, Yuzuru Tozawa. Establishment of a cell-free translation system from rice callus extracts. Bioscience, Biotechnology, and Biochemistry 84:2028-2036 (2020) doi.org/10.1080/09168451.2020.1779024.
Daniel Moog, Akira Nozawa, Yuzuru Tozawa, Ryoma Kamikawa. Substrate specificity of plastid phosphate transporters in a non-photosynthetic diatom and its implication in evolution of red alga-derived complex plastids. Scientific Reports 10:1167 (2020) doi.org/10.1038/s41598-020-58082-8

　Review, Interpretive article

Maki Komiya, Miki Kato, Daisuke Tadaki, Teng Ma, Hideaki Yamamoto, Ryugo Tero, Yuzuru Tozawa, Michio Niwano, Ayumi Hirano-Iwata. Advances in artificial cell membrane systems as a platform for reconstituting ion channels. Chemical Record 20:1-14 (2020)　
大島正弘, 戸澤譲, 加藤浩, HIS1共同研究グループ. イネにおけるトリケトン系除草剤ベンゾビシクロン(BBC)抵抗性と育種への展開. 植物の生長調節 55(2):131-136 (2020)

2019

　Original papers

Maeda H, Murata K, Sakuma N, Takei S, Yamazaki A, Karim MR, Kawata M, Hirose S, Kawagishi-K M, Taniguchi Y, Suzuki S, Sekino K, Ohshima M, Kato H, Yoshida H, Tozawa Y. A rice gene that confers broad-spectrum resistance to beta-triketone herbicides. Science 365: 393-396. doi: 10.1126/science.aax0379. (2019)

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Santos H, Imai K, Makiuchi T, Tomii K, Horton P, Nozawa A, Okada K, Tozawa Y, Nozaki T. Novel lineage-specific transmembrane β-barrel proteins in the endoplasmic reticulum of Entamoeba histolytica. FEBS J. doi: 10.1111/febs.14870 (2019)

2018

　Original papers

Imamura S, Nomura Y, Takemura T, Pancha I, Taki K, Toguchi K, Tozawa Y, Tanaka K. The checkpoint kinase TOR (target of rapamycin) regulates expression of a nuclear-encoded chloroplast RelA-SpoT homolog (RSH) and modulates chloroplast ribosomal RNA synthesis in a unicellular red alga. Plant Journal i2018 Feb 14. doi: 10.1111/tpj.13859. (2018)
Saito S, Hamamoto S, Moriya K, Matsuura A, Sato Y, Muto J, Noguchi H, Yamauchi S, Tozawa Y, Ueda M, Hashimoto K, Köster P, Dong Q, Held K, Kudla J, Utsumi T, Uozumi N. N-myristoylation and S-acylation are common modifications of Ca2+ regulated Arabidopsis kinases and are required for activation of the SLAC1 anion channel. New Phytologist Mar 2. doi: 10.1111/nph.15053. (2018)

2017

　Original papers

Tadaki D, Yamaura D, Araki S, Yoshida M, Arata K, Ohori T, Ishibashi K-I, Kato M, Ma T, Miyata R, Tozawa Y, Yamamoto H, Niwano M, Hirano-Iwata A. Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels. Scientific Reports 7: 17736 (2017)
Beljantseva J, Kudrin P, Jimmy S, Ehn M, Pohl R, Varik V, Tozawa Y, Shingler V, Tenson T, Rejman D, Hauryliuk V. Molecular mutagenesis of ppGpp: turning a RelA activator into an inhibitor. Scientific Reports 7: 41839 (2017)

2016

　Original papers

Yamashita S, Yamaguchi H, Waki T, Aoki Y, Mizuno M, Yanbe F, Ishii T, Funaki A, Tozawa Y, Miyagi-Inoue Y, Fushihara K, Nakayama T, Takahashi S. Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliensis. Elife 5: e19022 (2016)
Andresen L, Varik V, Tozawa Y, Jimmy S, Lindberg S, Tenson T, Hauryliuk V. Auxotrophy-based High Throughput Screening assay for the identification of Bacillus subtilis stringent response inhibitors. Scientific Reports 6: 35824 (2016)

2015

　Original papers

Mi-ichi F, Nozawa A, Yoshida H, Tozawa Y*, Nozaki T*. Evidence that Entamoeba histolytica mitochondrial carrier family links mitosomal and cytosolic pathways through exchange of PAPS and ATP. Eukaryotic Cell 14:1144-1150 (2015)
Watanabe S, Hiraoka Y, Endo S, Tanimoto Y, Tozawa Y, Watanabe Y. An Enzymatic method to estimate the content of L-hydroxyproline. Journal of Biotechnology 199:9-16 (2015)
Santos HJ, Imai K, Makiuchi T, Tomii K, Horton P, Nozawa A, Ibrahim M, Tozawa Y, Nozaki T. A Novel β-Barrel Mitosomal Outer Membrane Protein in Entamoeba. Scientific Reports 5:8545 (2015)

2014

　Original papers

Watanabe S, Tozawa Y, Watanabe Y. Ornithine cyclodeaminase/μ-crystallin homolog from hyperthermophilic archaeon Thermococcus litoralis functions as a novel Δ1-pyrroline-2-carboxylate reductase involved in putative trans-3-hydroxy-L-proline metabolism. FEBS Open Bio 4:617-626 (2014)
Nomura Y, Izumi A, Fukunaga Y, Kusumi K, Iba K, Watanabe S, Nakahira Y, Weber AMP, Nozawa A, Tozawa Y. Diversity in Guanosine 3',5'-Bisdiphosphate (ppGpp) Sensitivity Among Guanylate Kinases of Bacteria and Plants. Journal of Biological Chemistry 289:15631-15641 (2014)
Nomura Y, Nozawa A, Tozawa Y. Biochemical analyses of ppGpp effect on adenylosuccinate synthetases, key enzymes in purine biosynthesis in rice. Bioscience, Biotechnology, and Biochemistry 78:1022-1025 (2014)
Nozawa A, Tozawa Y. Incorporation of adenosine nucleotide transporter, ANT1, into proteoliposomes facilitates ATP translocation and activation of encapsulated luciferase. Journal of Bioscience and Bioengineering S1389-1723(14)00035-8. (2014)
Watanabe S, Tanimoto Y, Yamauchi S, Tozawa Y, Sawayama S, Watanabe Y. Identification and Characterization of trans-3-Hydroxy-L-proline Dehydratase and <DELTA>¹-Pyrroline-2-carboxylate Reductase Involved in trans-3-Hydroxy-L-proline Metabolism of Bacteria. FEBS Open Bio 4:240-250 (2014)

　Review, Interpretive article

野澤彰，戸澤譲．膜輸送タンパク質の完全インビトロ機能解析系．バイオサイエンスとインダストリー 72:(3) 190-196 (2014)
Nozawa A, Tozawa Y. Modifications of wheat germ cell-free system for functional proteomics of plant membrane proteins. Methods in Molecular Biology. 1072: 259-272 (2014)

2013

　Original papers

Nakahira Y, Ogawa A, Asano H, Oyama T, Tozawa Y. Theophylline-dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC7942. Plant Cell Physiology 54:1724-1735 (2013)
Nakahira Y, Ishikawa K, Tanaka k, Tozawa Y, Shiina T. Overproduction of hyperthermostable β-1,4-endoglucanase from the archaeon Pyrococcus horikoshii by tobacco chloroplast engineering. Bioscience, Biotechnology, and Biochemistry 77: 2140-2143 (2013)
Kore-eda S, Nozawa A, Okada Y, Nishiyama Y, Tozawa Y. Characterization of phosphoenolpyruvate/phosphate translocator from iceplant. Bioscience, Biotechnology, and Biochemistry 77:1511-1516 (2013)
Kubota S, Shirai O, Hibi T, Tozawa Y, Kano K. Effect of counter ions on the transport current across membranes containing KAT1 potassium channel. Analytical Sciences 29:161-164 (2013)

　Review, Proceedings

Bernhard F, Tozawa Y. Cell-Free Expression - Making a Mark. Current Opinion in Structural Biology. 23: 374-380 (2013)

2012

　Original papers

Watanabe S, Morimoto D, Fukumori F, Shinomiya H, Nishiwaki H, Kawano-Kawada M, Sasaki Y, Tozawa Y, Watanabe Y. Identification and characterization of D-Hydroxyproline dehydrogenase and Δ1-Pyrroline-4-Hydroxy-2-Carboxylate Deaminase involved in novel L-Hydroxyproline metabolism of bacteria: METABOLIC CONVERGENT EVOLUTION. Journal of Biological Chemistry 283:32674-32688 (2012)
Kato H, Watanabe S, Nimura-Matsune K, Chibazakura T, Tozawa Y, Yoshikawa H. Exploration of the possible partnership amoung orphan two-component system proteins in cyanobacterium Synechococcus elongates PCC 7942. Bioscience, Biotechnology, and Biochemistry 76:1484-1491(2012)
Yagi Y, Ishizaki Y, Nakahira Y, Tozawa Y, Shiina T. Non-prokaryotic nucleoid protein pTAC3 is essential for transcription by a cyanobacteria-derived RNA polymerase in chloroplasts. Proceedings of the National Academy of Sciences of the United States of America 109:7541-7546 (2012)
Tagami K, Nanamiya H, Kazo Y, Maehashi M, Suzuki S, Namba E, Tozawa Y, Morimoto T, Ogasawara N, Kageyama Y, Ara K, Ozaki K, Yoshida M, Kuroiwa T, Ohashi Y, Kawamura F. Expression of small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome. MicrobiologyOpen 1:115-134 (2012)
Nomura Y, Takabayashi T, Kuroda H, Yukawa Y, Sattasuk K, Akita M, Nozawa A, Tozawa Y. ppGpp inhibits peptide elongation cycle of chloroplast translation system in vitro. Plant Molecular Biology 78:185-196 (2012)

　Review, Interpretive article

Tozawa Y. Cell-free protein synthesis of membrane-integrated proteins for functional analysis. (2011) Frontiers in Agriculture Proteome Research, Contribution of Proteomics Technology in Agricultural Sciences, Proceedings of the 3rd International Symposium on “Frontiers in Agriculture Proteome Research”, Eds., Mock HP, Wang ZY, and Komatsu S. 16-23 (2012)

2011

　Original papers

Sattasuk K, Nozawa A, Tozawa Y, Kakinuma Y, Akita M. In vitro protein import of a putative amino acid transporter from Arabidopsis thaliana into chloroplasts and its suborganellar localization. Bioscience, Biotechnology, and Biochemistry 75:2200-2006 (2011)
Nozawa A, Fujimoto R, Matsuoka H, Tsuboi T, Tozawa Y. Cell-free protein synthesis, reconstitution and characterization of mitochondrial dicarboxylate-tricarboxylate carrier of Plasmodium falciparum. Biochemical and Biophysical Research Communications 414:612-617 (2011)
Tozawa Y, Nomura Y. Signaling by the global regulatory molecule ppGpp in bacteria and chloroplasts of land plants. Plant Biolology 13:609-709 (2011)
Narimatsu Y, Kubota T, Furukawa S, Shimojima M, Iwasaki H, Tozawa Y, Tachibana K, Narimatsu H. Co-translational function of Cocmc, core 1 synthase specific molecular chaperine, revealed by a cell-free translation system. FEBS Letters 585:1276-1280 (2011)
Ito K, Nozaki M, Ohta T, Miura C, Tozawa Y, Miura T. Differences of two polychaete species reflected in enzyme activities. Marine Biology 158:1211-1221 (2011)
Ohta T, Ueda Y, Ito K, Celino FT, Miura C, Yamashita H, Miura T, Tozawa Y. Anti-viral effects of interferon administration on Sevenband grouper, Epinephelus septemfasciatus. Fish Shellfish Immunology 30:1064-1071 (2011)
Celino FT, Yamaguchi S, Miura C, Ohta T, Tozawa Y, Iwai T, Miura T. Tolerance of spermatogonia to oxidatve stress is due to high levels of Zn and Cu/Zn superoxide dismutase. PLoS ONE 6:e16938 (2011)

2010

　Original papers

Genji T, Nozawa A, and Tozawa Y. Efficient production and purification of functional bacteriorhodopsin with a wheat-germ cell-free system and a combination of Fos-choline and CHAPS detergents. Biochemical and Biophysical Research Communications 400:638-642 (2010)
Chadani Y, Ono K, Ozawa S-I, Takahashi Y, Takai K, Nanamiya H, Tozawa Y, Kutsukake K, and Abo T. Ribosome rescue by Escherichia coli AffA (YhdL) in the absence of trans-translation system. Molecular Microbiology 78:796-808 (2010)
Yamauchi S, Fusada N, Hayashi H, Utsumi T, Uozumi N, Endo Y, and Tozawa Y. The concensus motif for N-myristoylation of plant proteins in a wheat germ cell-free translation system. FEBS Journal 277:3596-3607 (2010)
Nishiyama Y, Yun CS, Masuda F, Sasaki T, Saito K, and Tozawa Y. Expression of bacterial tyrosine ammonia-lyase creates a novel p-coumaric acid pathway in the biosynthesis of phenylpropanoids in Arabidopsis thaliana. Planta 232 (1):201-218 (2010)
Ohta T, Matsuoka H, Nomura Y, and Tozawa Y. Control of translational initiation in the wheat-embryo cell-free protein expression system for producing homogenous products. Protein Expression and Purification 73:15-22 (2010)

　Review, Interpretive article

戸澤譲，笠井光治．高等植物におけるppGpp合成系とその生理学的機能の解明をめざして. 植物の生長調節 45(2):119-124 (2010)
Nozawa A, Nanamiya H, and Tozawa Y. Production of membrane proteins through the wheat-germ cell-free technology. Methods in Molecular Biology 607:213-218 (2010)
Kanno T, and Tozawa Y. Protein engineering accelerated by cell-free technology. Methods in Molecular Biology 607:85-99 (2010)

2009

　Original papers

Natori Y, Tagami K, Murakami K, Yoshida S, Tanigawa O, Masuda K, Wada T, Suzuki S, Nanamiya H, Tozawa Y, and Kawamura F. Transcription activity of individual rrn operons in Bacillus subtilis mutants deficient of (p)ppGpp synthetase genes, relA, yjbM, and ywaC. Journal of Bacteriology 191 (14):4555-4561 (2009)
Nozaki M, Miura C, Tozawa Y, and Miura T. The contribution of endogenous-cellulose digestion in the gut of earthworm (Pheretima hilgendorfi:Megascolecidae). Soil Biology and Biochemistry 41:762-769 (2009)
Yun CS, Hasegawa H, Terakawa T, and Tozawa Y. Novel bacterial N-acetyltransferase gene for herbicide detoxification in land plants and selectable marker in plant transformation. Bioscience, Biotechnology, and Biochemistry 73(5):1000-1006 (2009)

　Review, Interpretive article

野澤彰，戸澤譲，澤崎達也，遠藤弥重太．コムギ無細胞翻訳系を用いた膜タンパク質生産．蛋白質核酸酵素 54:(12) 1443-1447 (2009)
戸澤譲，野澤彰，源治尚久．無細胞翻訳系を利用した膜タンパク質の機能解析．化学と生物 47:(2) 98-103 (2009)

2008

　Original papers

Masuda S, Tozawa Y, and Ohta H. Possible targets of "magic spots" in plant signaling. Plant Signaling & Behavior 3 (11): 1021-1023 (2008)
Yamada T, Matsuda F, Kasai K, Fukuoka S, Kitamura K, Tozawa Y, Miyagawa H, and Wakasa K. Mutation of an enzyme that functions in phenylalanine biosynthesis results in accumulation of phenylalanine and tryptophan in rice. Plant Cell 20 (5):1316-1329 (2008)
Yun CS, Yamamoto T, Nozawa A, Tozawa Y. Expression of parsley flavone synthase I establishes the flavone biosynthetic pathway in Arabidopsis thaliana. Bioscience, Biotechnology, and Biochemistry 72 (4): 968-973 (2008)
Masuda S, Mizusawa K, Narisawa T, Tozawa Y, Ohta H, and Takamiya KI. The Bacterial Stringent Response, Conserved in Chloroplasts, Controls Plant Fertilization. Plant Cell Physiology 49 (2):135-141 (2008)
Nanamiya H, Kasai K, Nozawa A, Yun C-S, Narisawa T, Murakami K, Natori Y, Kawamura F, and Tozawa Y. Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis. Molecular Microbiology 67 (2): 291-304 (2008)

　Review, Interpretive article

戸澤譲，笠井光治，七宮英晃．高等植物とバクテリアにおける多様なppGpp合成系とその生理学的機能．バイオサイエンスとインダストリー 66:293-297 (2008)
Nozaki M, Miura C, Tozawa Y, and Miura T. The role of the earthworm, Pheretima (Metaphire) holgendorfi, in terrestrial ecosystem nutrient cycling. Interdisciplinary Studies on Environmental Chemistry–Biological Responses to Chemical Pollutants, Eds., Murakami Y, Nakayama N, Kitamura S-I, Iwata H, and Tanabe S. 275-279 (2008)
Tagami K, Wada T, Maehashi M, Hirohata Y, Yoshida M, Kuroiwa H, Kuroiwa T, Nanamiya H, Tozawa Y, Liu S, Kageyama Y, Ara K, Ozaki K, Kawamura F. Functional analysis of a novel ppGpp synthetase, YwaC, and regulatory mechanism for the dimerization of ribosome in Bacillus subtilis. Genes & Genetics Systems 83:513 (2008)
Tagami K, Wada T, Yoshida M, Kuroiwa H, Kuroiwa T, Nanamiya H, Tozawa Y, Kawamura F. Functional analysis of a novel ppGpp synthetase, YwaC, in Bacillus subtilis. Genes & Genetics Systems 83:513 (2008)

2007

　Original papers

Nozawa A, Nanamiya H, Mityata T, Linka N, Endo Y, Weber APM, and Tozawa Y. A cell-free translation and proteoliposome-reconstitution system for functional analysis of plant solute transporters. Plant Cell Physiology 48 (12): 1815-1820 (2007)
Tozawa Y, Nozawa A, Kanno T, Narisawa T, Masuda S, Kasai K, Nanamiya, H. Calcium-activated (p)ppGpp synthetase in chloroplasts of land plants. Journal of Biological Chemistry 282 (49): 35536-35545 (2007)
Tozawa Y, Teraishi M, Sasaki T, Sonoike K, Nishiyama Y, Itaya M, Miyao A, and Hirochika H. The plastid sigma factor SIG1 maintains photosystem I activity via regulated expression of the psaA operon in rice chloroplasts. Plant Journal 52: 124-132 (2007)
Kojima K, Oshita M, Nanjo Y, Kasai K, Tozawa Y, Hayashi H, and Nishiyama Y. Oxidation of elongation factor G inhibits the synthesis of the D1 protein of photosystem II. Molecular Microbiology 64: 936-947 (2007)
Kubota Y, Miyao A, Hirochika H, Tozawa Y, Yasuda H, Tsunoyama Y, Niwa Y, Imamura S, Shirai M, and Asayama M. Two novel nuclear genes, OsSIG5 and OsSIG6, encoding potential plastid sigma factors of RNA polymerase in rice: tissue-specific and light-responsive gene expression. Plant Cell Physiol.48: 186-192 (2007)
Kanno T, Kitano M, Kato R, Ohmori A, Endo Y, and Tozawa Y. Sequence specificity and efficiency of protein N-terminal methionine elimination in wheat-embryo cell-free system. Protein Expression and Purification 52: 59-65 (2007)
Kuroita T, Kanno T, Kawai A, Kawakami B, Oka M, Endo Y, and Tozawa Y. Functional similarities of a thermostable protein-disulfide oxidoreductase identified in the archaeon Pyrococcus horikoshii to bacterial DsbA enzymes. Extremophiles 11: 85-94 (2007)

　Proceedings

Kasai K, Obayashi Y, Yamada T, Kanno T, Wakasa K, Tozawa Y. Functional analysis of arogenate dehydratase of rice by means of wheat-embryo cell-free protein expression system. Plant Cell Physiol.48:S219 (2007)
Tozawa Y, Teraishi M, Sasaki T, Sonoike K. Functional roles of plastid SIG1 for gene expression of photosystem I components. Plant Cell Physiology 48:S35 (2007)

2006

　Original papers

Wakasa K, Hasegawa H, Nemoto H, Matusda F, Miyazawa H, Tozawa Y, Morino K, Komatsu A, Yamada T, Terakawa T, Miyagawa H. High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile. Journal of Experimental Botany 57: 3069-3078 (2006)
Ozaki Y, Higuchi M, Miura C, Yamaguchi S, Tozawa Y, and Miura T. Roles of 11b-hydroxysteroid dehydrogenase in fish spermatogenesis. Endocrinology 147: 5139-5146(2006)
Makino SI, Sawasaki T, Tozawa Y, Endo Y, and Takai K. Covalent circularization of exogenous RNA during incubation with a wheat embryo cell extract. Biochemical and Biophysical Research Communications 347: 1080-1087 (2006)
Hirano N, Sawasaki T, Tozawa Y, Endo Y, and Takai K. Tolerance for random recombination of domains in prokaryotic and eukaryotic translation systems: limited inter-domain misfolding in a eukaryotic translation system. Proteins 64: 343-354 (2006)

　Proceedings

Murakami K, Natori Y, Sato M, Nanamiya H, Kawamura F, Tozawa Y. Function analysis of relA and it’s paralogous genes, yjbM and ywaC, in Bacillus subtilis. Genes & Genetics Systems 81:442 (2006)

2005

　Original papers

Kanno T, Komatsu A, Kasai K, Dubouzet JG, Sakurai M, Ikejiri-K Y, Wakasa K, and Tozawa Y. Structure-based in vitro engineering of the anthranilate synthase, a metabolic key enzyme in the plant Trp biosynthetic pathway. Plant Physiology 138: 2260-2268 (2005)
Kasai K, Kanno T, Akita M, Ikejiri-Kanno Y, Wakasa K, and Tozawa Y. Identification of three shikimate kinase genes in rice: characterization of their differential expression during panicle development and of the enzymatic activities of the encoded proteins. Planta 222:438-447 (2005)

　Review, Interpretive article

戸澤譲．進化する無細胞タンパク質合成技術．BIONICS MAY 2005: 44-49 (2005)
戸澤譲，越智幸三．葉緑体(p)ppGpp合成酵素．蛋白質核酸酵素 50:(14) 1860 (2005)
Sawasaki T, Gouda MD, Kawasaki T, Tsuboi T, Tozawa Y, Takai K, and Endo Y. The Wheat Germ Cell-Free Expression System. Methods in Molecular Biology 310:131-144 (2005)

2004

　Original papers

Kasai K, Kanno T, Wakasa K, Endo Y, and Tozawa Y. Guanosine tetra- and pentaphosphate synthetase activity in chloroplasts of a higher plant: association with 70S ribosomes and inhibition by tetracycline. Nucleic Acids Research 32: (19) 5732-5741 (2004)
Kusumi K, Yara A, Mitsui N, Tozawa Y, and Iba K. Characterization of a rice nuclear-encoded plastid RNA polymerase gene OsRpoTp. Plant Cell Physiology 45: (9) 1194-1201 (2004)
Yamada T, Tozawa Y, Hasegawa H, Terakawa T, Ohkawa Y, and Wakasa K. Use of a feedback-insensitive a subunit of anthranilate synthase as a selectable marker for transformation of rice and potato. Molecular Breeding 14: 363-373 (2004)
Sugimoto H, Kusumi K, Tozawa Y, Yazaki J, Kishimoto N, Kikuchi S, and Iba K. The virescent-2 Mutation Inhibits Translation of Plastid Transcripts for the Plastid Genetic System at an early stage of chloroplast differentiation. Plant Cell Physiology 45: (8)985-996 (2004)
Tanaka N, Fujita M, Handa H, Muryama S, Umemura M, Kawamura Y, Mitsui T, Mikami S, Tozawa Y, Yoshinaga T, and Komatsu S. Proteomics of the rice cell: systematic identification of the protein population in subcellular compartments. Molecular Genetics and Genomics 271: (5) 566-576 (2004)
Kanno T, Kasai K, Ikejiri-Kanno Y, Wakasa K, and Tozawa Y. In vitro reconstitution of rice anthranilate synthase: distinct functional properties of the a subunits OASA1 and OASA2. Plant Molecular Biology 54: (1) 11-23 (2004)
Kasai K, K-Kobayashi M, Teraishi M, Ito Y, Ochi K, Wakasa K, and Tozawa Y. Differential Expression of Three Plastidial Sigma Factors, OsSIG1, OsSIG2A and OsSIG2B, during leaf development in rice. Bioscience, Biotechnology, and Biochemistry 68: (4) 973-977 (2004)

　Review, Proceedings

Ochi K, Okamoto S, Tozawa Y, Inaoka T, Hosaka T, Xu J, and Kurosawa K. Ribosome Engineering and Secondary Metabolite Production. Advances in Applied Microbiology Review 56: 155- 184 (2004)
Sugimoto H, Kusumi K, Yoshimura A, Kikuchi S, Tozawa Y, Iba K. Functional analysis of rice virescent-2 gene essential for chloroplast development. Plant Cell Physiology 45:S208 (2004)

2003

　Original papers

Ohashi Y, Inaoka T, Kasai K, Ito Y, Okamoto S, Satsu H, Tozawa Y, Kawamura F, and Ochi K. Expression Profiling of Translation-associated Genes in Sporulation Bacillus subtilis and Consequence of Sporulation by Gene Inactivation. Bioscience, Biotechnology, and Biochemistry 67: (10) 2245-2253 (2003)

　Review, Interpretive article

若狭暁、戸澤譲. トリプトファン生合成における一次・二次代謝の制御と利用をめざして. 植物の生長調節38:249-254 (2003)
Tozawa Y. “Magic spot” synthase in chloroplasts. 日本生化学会誌ミニレビュー 75:600-604 (2003)
Tozawa Y, Sawasaki T, and Endo Y. Cell-free Protein Synthesis by Wheat Germ Extracts. 日本結晶学会誌総説, 45:3-8 (2003)
Kusumi K, Kouno T, Sugimoto H, Tozawa Y, Yoshimura A, Kikuchi S, Iba K. Expression analysis of chloroplast proteins in virescent mutants (v(1), v(3)) of rice. Plant Cell Physiol. 44:S57 (2003)

~2002

　Original papers

Ito Y, Ozawa A, Sawasaki T, Endo Y, Ochi K, and Tozawa Y. OsRALyase1, a Putative F-Box Protein Identified in Rice Oryza sativa, Possesses Enzyme Activity Identical to That of Wheat RALyase. Bioscience, Biotechnology, and Biochemistry 66: 2725-2729 (2002)
Kasai K, Usami S, Yamada T, Endo Y, Ochi K, and Tozawa Y. A RelA-SpoT homolog (Cr-RSH) identified in Chlamydomonas reinhardtii generates stringent factor in vivo and localizes to chloroplasts in vitro. Nucleic Acids Research 30: 4985-4992 (2002)
Xu J, Tozawa Y, Lai C, Hyashi H, and Ochi K. Rifampicin resistant mutation in the rpoB gene confers ppGpp-independent antibiotic production to Streptomyces coelicolor A3(2). Molecular and General Genetics 268: 179-189 (2002)
Lai C, Xu J, Tozawa Y, Okamoto-H Y, Yao X, and Ochi K. Genetic and physiological characterization of rpoB mutations which activate antibiotic production in Streptomyces lividans. Microbiology 148: 3365-3373 (2002)
Tozawa Y, Hasegawa H, Terakawa T, and Wakasa K. Characterization of rice anthranilate synthase alfa subunit genes OSASA1 and OSASA2: tryptophan accumulation in transgenic rice expressing a feedback-insensitive mutant of OASA1. Plant Physiology 126: 1493-1506 (2001)
Urushibara S, Tozawa Y, Kawagishi-Kobayashi M, and Wakasa K. Efficient transformation of rice suspension-cultured cells mediated by Agrobacterium tumefaciens. Breeding Science 51: 33-38 (2001)
Asada A, Zhao Y, Komano H, Kuwata T, Mukai M, Fujita K, Tozawa Y, Iseki R, Tian H, Sato K, Motegi K, Suzuki R, Yokoyama M, and Iwata M. The calcium-independent protein kinase C participates in an early process of CD3/CD28-mediated induction of thymocyte apoptosis. Immunology 101: 309-315 (2000)
Tsunoyama Y, Tozawa Y, and Wakasa K. Coexistence of the plastidial sigma factor SigA and bacterial RNA polymerase activity in partially purified chloroplast soluble protein fraction. Rice Genetics Newsletter 15:176-178 (1999)
Tozawa Y, Tanaka K, Takahashi H, and Wakasa K. Nuclear encoding of a plastid sigma factor in rice and its tissue- and light-dependent expression. Nucleic Acids Research 26:415-419 (1998)
Tanaka K, Tozawa Y, Mochizuki N, Shinozaki K, Nagatani A, Wakasa K, and Takahashi H. Characterization of three cDNA species encoding plastid RNA polymerase sigma factors in Arabidopsis thaliana: evidence for the sigma factor heterogeneity in higher plant plastids. FEBS Letters 413:309-313 (1997)
Iwata M, Kuwata T, Mukai M, Tozawa Y, and Yokoyama Y. Differential induction of helper and killer T cells from isolated CD4+CD8+ thymocytes in suspension culture. European Journal of Immunology 26:2081-2086 (1996)
Ohoka Y, Kuwata T, Tozawa Y, Zhao Y, Mukai M, Motegi Y, Suzuki R, Yokoyama M, and Iwata M. In vitro differentiation and commitment of CD4+ CD8+ thymocytes to the CD4 lineage, without TCR engagement. International Immunology 8:297-306 (1996)
Sato TN, Tozawa Y, Deutsch U, Wolburg-Buchholz K, Fujiwara Y, Gendron-Maguire M, Gridley T, Wolburg H, Risau W, and Qin Y. Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation. Nature 376: 70-74 (1995)
Zhao Y, Tozawa Y, Iseki R, Mukai M, and Iwata M. Calcineurin activation protects T cells from glucocorticoid-induced apoptosis. Journal of Immunology 154:6346-6354 (1995)
Iwata M, Iseki R, Sato K, Tozawa Y, and Ohoka Y. Involvement of protein kinase C-epsilon in glucocorticoid-induced apoptosis in thymocytes. International Immunology 6:431-438 (1994)
Tozawa Y, Yoshikawa H, Kawamura F, Itaya M, and Takahashi H. Isolation and characterization of the groES and groEL genes of Bacillus subtilis Marburg. Bioscience, Biotechnology, and Biochemistry 56:1995-2002 (1992)
Yamazaki S, Kobayashi N, Okubo A, Sato T, Tozawa Y, Yoshimura E, Kajiwara H, Hirano H, and Toda S. Charactreization of dimethylsulfoxide reductase, a novel molybdo-enzyme from photosynthetic bacteria, Rhodobacter spheroids f.s. denitrificans. Analytical Sciences 7:821-824 (1991)

　Review, Interpretive article

Komatsu A, Yoshikawa A, Hasegawa H, Kawagishi-Kobayashi M, Nishizawa Y, Sugimoto K, Tozawa Y, Wakasa K. Analysis of rice transformants with the mutated anthranilate synthase gene driven by five different promoters fro the development of selection marker. Plant Cell Physiology 43:S121 (2002)
Tozawa Y, Tanaka K, Wakasa K. Molecular cloning of the gene encoding putative chloroplast RNA polymerase sigma subunit from rice. Plant Physiology 114:1276 (1997)
Crouch RJ, Tozawa Y, and Cerritelli SM. Ribonuclease H of Saccharomyces pombe and Crithithidia fasciculate share a common moth in their non-RNase-H domain that binds double-stranded-RNA in a magnesium-dependent manner. Protein Engineering 7:1151-1152 (1994)
Tozawa Y, Sato TN. Roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation. EXPERIMENTAL MEDICINE (実験医学) 14:88-91 (1996)