植物水ストレス生理学ユニット

• Karim, M. M., Islam, M., Miyabe, M. T., Ikeda, Y., Polash, M. A. F., Hirota, K., Sakayama, H., Sakata, Y. and Takezawa, D. (2025) ETHYLENE INSENSITIVE2-like protein mediates submergence and drought responses in Physcomitrium patens.Plant Physiol., kiaf293.
• Jinno, C., Fujisaki, K., Yotsui, I., Ouchi, M., Singh, P., Naramoto, S., Takezawa, D., Sakata, Y. and Fujita, T. (2025) Abscisic acid signaling regulates primary plasmodesmata density for plant cell-to-cell communication. Sci. Adv., 11: eadr8298.
• Miyabe, M.T., Sasaki, T., Toriyama, T., Sk, R., Takezawa, D., Yotsui, I., Taji, T., and Sakata, Y. (2025) Evolution and divergence of the role of plant ethylene receptor-related histidine kinases in abscisic acid signaling. Biochem. Biophys. Res. Commun. 747:151295.
• Khatun, N., Shinozawa, A., Kosaku, T., Matsuura, H., Jahan, A., Islam, M., Karim, M. M., Sk, R., Yoshikawa, M., Ishizaki, K., Sakata, Y., Takezawa, D. (2023)Abscisic acid-mediated sugar responses are essential for vegetative desiccation tolerance in the liverwort Marchantia polymorpha.Physiologia Plantarum., 175(2):e13898
• Sk, R., Miyabe, M.T., Takezawa, D., Yajima, S., Yotsui, I., Taji, T., Sakata, Y. (2022) Targeted in vivo mutagenesis of a sensor histidine kinase playing an essential role in ABA signaling of the moss Physcomitrium patens. Biochem. Biophys. Res. Commun., 637: 93-99.
• Jahan, A., Yamazaki, Y., Islam, M., Ghosh, T. K., Yoshimura, N., Kato, H., Ishizaki, K., Shinozawa, A., Sakata, Y., Takezawa, D. (2022)Differential regulations of abscisic acid-induced desiccation tolerance and vegetative dormancy by group B3 Raf kinases in liverworts. Front. Plant Sci., 13:952820.
• Toriyama, T., Shinozawa, A., Yasumura, Y., Saruhashi, M., Hiraide, M., Ito, S., Matsuura, H., Kuwata, K., Yoshida, M., Baba, T., Yotsui, I., Taji, T., Takezawa, D. and Sakata, Y. (2022) Sensor histidine kinases mediate ABA and osmostress signaling in the moss Physcomitrium patens. Curr. Biol., 32(1):164-175.e8.
• Kamiyama, Y., Hirotani, H., Ishikawa, S., Minegishi, F., Katagiri, S., Rogan, C.J., Takahashi, F., Nomoto, M., Ishikawa, K., Kodama, Y., Tada, Y., Takezawa, D., Anderson, J.C., Peck, S.C., Shinozaki, K. and Umezawa, T. (2021) Arabidopsis Group C Raf-like protein kinases negatively regulate abscisic acid signaling and are direct substrates of SnRK2. Proc. Natl. Acad. Sci. U. S. A., 118(30): e2100073118.
• Islam, M., Inoue, T., Hiraide, M., Khatun, N., Jahan, A., Kuwata, K., Katagiri, S., Umezawa, T., Yotsui, I., Sakata, Y. and Takezawa, D. (2021) Activation of SnRK2 by Raf-like kinase represents a primary mechanism of ABA and abiotic stress responses. Plant Physiol., 185(2): 533–546.
• Katsuta, S., Masuda, G., Bak, H., Shinozawa, A., Kamiyama, Y., Umezawa, T., Takezawa, D., Yotsui, I., Taji, T. and Sakata, Y. (2020) Arabidopsis Raf-like kinases act as positive regulators of subclass III SnRK2 in osmostress signaling.　Plant J., 103(2):634-644.
• Komatsu, K., Takezawa, D. and Sakata, Y. (2020) Decoding ABA and osmostress signalling in plants from an evolutionary point of view. Plant Cell Environ., 43(12):2894-2911.
• Jahan, A., Komatsu, K., Wakida-Sekiya, M., Hiraide, M., Tanaka, K., Ohtake, R., Umezawa, T., Toriyama, T., Shinozawa, A., Yotsui, I., Sakata, Y. and Takezawa, D. (2019) Archetypal roles of the PYR/PYL/RCAR-like receptor in drought and sugar responses in liverworts. Plant Physiol., 179(1):317-328.
• Shinozawa, A., Otake, R., Takezawa, D., Umezawa, T., Komatsu, K., Tanaka, T., Amagai, A., Ishikawa, S., Hara, Y., Kamisugi, Y., Cuming, A.C., Hori, K., Ohta, H., Takahashi, F., Shinozaki, K., Hayashi, T., Taji, T. and Sakata, Y. (2019) SnRK2 protein kinases represent an ancient system in plants for adaptation to a terrestrial environment. Commun. Biol., 2:30.
• Amagai, A., Honda, Y., Ishikawa, S., Hara, Y., Takezawa, D., Sakata, Y., Shinozaki. K. and Umezawa, T. (2018) Phosphoproteomic profiling reveals ABA-responsive phosphosignaling pathways in Physcomitrella patens. Plant J., 94(4):699-708.
• Takezawa, D. (2018) Mechanisms underlying freezing and desiccation tolerance in bryophytes. Survival Strategies in Extreme Cold and Desiccation, Springer., pp167-187.

光合成微生物ユニット

• Kubodera, H., Inoue, H., Ando, A., Takahashi, T., Hihara, Y. (2026) SOS response and functional diversification of the transcription factor LexA in cyanobacteria. Plant Physiol., in press
• Ogawa, T., Takahashi, H., Nishiyama, Y., Hihara, Y., Sonoike, K. (2025) Light-induced increase in the steady-state chlorophyll fluorescence in cyanobacteria reflects induction of energy dissipation complementary to orange carotenoid protein-dependent thermal dissipation.  Photosynth. Res.,163: article number 38
• Tanikawa, R., Sakaguchi, H., Ishikawa, T., Hihara, Y. (2025) Accumulation of acyl plastoquinol and triacylglycerol in six cyanobacterial species with different sets of genes encoding type-2 diacylglycerol acyltransferase-like proteins. Plant Cell Physiol., 66(1):15-22.
• Nakamura, R., Takahashi, Y., Tachibana, S., Terada, A., Suzuki, K., Kondo, K., Tozawa, Y., Hihara, Y. (2024) Partner-switching components PmgA and Ssr1600 regulate high-light acclimation in Synechocystis sp. PCC 6803. Plant Physiol.,196(1):621-633.
• Hishida, A., Shirai, R., Higo, A., Matsutani, M., Nimura-Matsune, K., Takahashi, T., Watanabe, S., Ehira, S., Hihara, Y. (2024) CRISPRi knockdown of the cyabrB1 gene induces the divergently transcribed icfG and sll1783 operons related to carbon metabolism in the cyanobacterium Synechocystis sp. PCC 6803.J. Gen. Appl. Microbiol., 70(1): doi: 10.2323/jgam.2024.01.001.
• Ishikawa, T., Takano, S., Tanikawa, R., Fujihara, T., Atsuzawa, K., Kaneko, Y., Hihara, Y. (2023) Acylated plastoquinone is a novel neutral lipid accumulated in cyanobacteria. PNAS Nexus 2(5): pgad092.
• Kato, N., Iwata, K., Kadowaki, T., Sonoike, K., Hihara Y. (2022) Dual redox regulation of the DNA-binding activity of the response regulator RpaB in the cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol., 63: 1078-1090.
• Ishikawa, Y., Cassan, C., Kadeer, A., Yuasa, K., Sato, N., Sonoike, K., Kaneko, Y., Miyagi, A., Takahashi, H., Ishikawa, T., Yamaguchi, M., Nishiyama, Y., Hihara, Y., Gibon, Y., Kawai-Yamada, M. (2021) The NAD kinase Slr0400 functions as a growth repressor in Synechocystis sp. PCC 6803. Plant Cell Physiol., 62: 668-677.
• Kawahara, A. and Hihara, Y. (2021) Biosynthesis of fatty acid derivatives by cyanobacteria: From basics to biofuel production. In Cyanobacteria Biotechnology (Edited by Hudson P)., pp.331-368, Wiley.
• Takashima, K., Nagao, S., Kizawa, A., Suzuki, T., Dohmae, N. and Hihara, Y. (2020) The role of transcriptional repressor activity of LexA in salt-stress responses of the cyanobacterium Synechocystis sp. PCC 6803. Sci. Rep., 10 (1):17393.
• Tanaka, M., Ishikawa, T., Tamura, S., Saito, Y., Kawai-Yamada, M. and Hihara, Y. (2020) Quantitative and qualitative analyses of triacylglycerol production in the wild-type cyanobacterium Synechocystis sp. PCC 6803 and the strain expressing AtfA from Acinetobacter baylyi ADP1. Plant Cell Physiol., 61(9):1537-1547.
• Jimbo, H., Izuhara, T., Hihara, Y., Hisabori, T., Nishiyama, Y. (2019) Light-inducible expression of translation factor EF-Tu during acclimation to strong light enhances the repair of photosystem II. Proc. Natl Acad. Sci. U S A., 116: 21268-21273.
• Higo, A., Nishiyama, E., Nakamura, K., Hihara, Y., Ehira, S. (2019) cyAbrB Transcriptional regulators as safety devices to inhibit heterocyst differentiation in Anabaena sp. Strain PCC 7120. J. Bacteriol., 201: pii: e00244-19.
• Riediger, M., Kadowaki, T., Nagayama, R., Georg, J., Hihara, Y. and Hess, W.R. (2019) Biocomputational analyses and experimental validation identify the complex regulon controlled by the redox-responsive transcription factor RpaB. iScience., 15: 316-331.
• Ishikawa, Y., Miyagi, A., Ishikawa, T., Nagano, M., Yamaguchi, M., Hihara, Y., Kaneko, Y., Kawai-Yamada, M. (2019) One of the NAD kinases, sll1415, is required for the glucose metabolism of Synechocystis sp. PCC 6803. Plant J., 98: 654-666.
• Kodama, Y., Kawahara, A., Miyagi, A., Ishikawa, T., Kawai-Yamada, M., Kaneko, Y., Takimura, Y. and Hihara, Y. (2018) Effects of inactivation of the cyAbrB2 transcription factor together with glycogen synthesis on cellular metabolism and free fatty acid production in the cyanobacterium Synechocystis sp. PCC 6803. Biotechnol. Bioeng., 115(12): 2974-2985.
• Kujirai, J., Nanba, S., Kadowaki, T., Oka, Y., Nishiyama, Y., Hayashi, Y., Arai, M. and Hihara, Y. (2018) Interaction of the GntR-family transcription factor Sll1961 with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803. Sci. Rep., 8 (1): 6666.
• Jimbo, H., Yutthanasirikul, R., Nagano, T., Hisabori, T., Hihara, Y., Nishiyama, Y. (2018) Oxidation of translation factor EF-Tu inhibits the repair of photosystem II. Plant Physiol., 176: 2691-2699.
• Riediger, M., Hihara, Y. and Hess W.R. (2018) From cyanobacteria and algae to land plants: The RpaB/Ycf27 regulatory network in transition. Perspect Phycol., 5 (1): 13-25.

植物バイオマスユニット

• Yamaguchi M., Sato A., Takahashi D., Mori K., Fujimoto R., Miyagi A., Sato E., Ishikawa T., Sano R., Kurata T., Suzuki S., Kaneko Y., Kawai-Yamada M., Kotake T. (2025) The rice BRITTLE CULM 4 gene encodes a membrane protein involved in cellulose synthesis in the secondary cell wall. Plant Cell Physiol. 66: 1444-1453.
• Kikuchi T., Soga K., Kotake T., Takahashi D. (2025) Defective pollen meiosis in Arabidopsis due to combined arabinan and galactan insufficiency. Plant Cell Physiol. 66: 1346-1359.
• Yoshimi Y., Yu L., Cresswell R., Guo X., Echevarria-Poza A., Lyczakowski J.J., Dupree R., Kotake T., Dupree P. (2025) Glucomannan engineering highlights the role of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls. Nat. Commun. 16: 1235.
• Nakamura K., Kikuchi Y., Shiraga M., Kotake T., Taketa S., Ikeda Y. (2025) SHORT AND CROOKED AWN, encoding an epigenetic regulator EMF1, promotes barley awn development. Plant Cell Physiol. 66: 705-721.
• Umezawa, A., Matsumoto, M., Handa, H., Nakazawa, K., Miyagawa, M., Seifert, G.J., Takahashi, D., Fushinobu, S., Kotake, T. (2024) Cytosolic UDP-L-arabinose synthesis by bifunctional UDP-glucose 4-epimerases in Arabidopsis. Plant J. 119: 508-524.
• Takahashi, D., Soga, K., Kikuchi, T., Kutsuno, T., Hao, P.F., Sasaki, K., Nishiyama, Y., Kidokoro, S., Sampathkumar, A., Bacic, A., Johnson, K.L., Kotake T. (2024) Structural changes in cell wall pectic polymers contribute to freezing tolerance induced by cold acclimation in plants. Curr. Biol. 34: 958-968.
• Tokuyasu, K., Yamagishi, K., Kotake, T., Kimura, S., Ike, M. (2024) Hydrogen chloride treatment of rice straw for upcycling into nanofibrous products for sugar pool. Biores. Technol. Rep. 25: 101717.
• Kotake, T., Takahashi, D. (2024) On the separate metabolisms of UDP-sugars and GDP-sugars in plants. Trends Glycosci. Glycobiol. 36: E6-E11.
• Mukae, K., Md. Sakil, A., Kotake, T., Inoue-Aono, Y., Moriyasu, Y. (2023) Autophagy accelerates cell death after desiccation and hydration stress in Physcomitrium. Environ. Exp. Bot. 213: 105412.
• Kikukawa, K., Takigawa-Imamura, H., Soga, K., Kotake, T., Higaki, T. (2023) Smooth elongation of pavement cells induced by RIC1 overexpression leads to marginal protrusions of the cotyledon in Arabidopsis thaliana. Plant Cell Physiol. 64: 1356-1371.
• Yoshida, H., Okada, S., Wang, F., Shiota, S., Mori, M., Kawamura, M., Zhao, X., Wang, Y., Nishigaki, N., Kobayashi, A., Miura, K., Yoshida, S., Ikegami, M., Ito, A., Huang, L.T., Caroline, Hsing, Y.I., Yamagata, Y., Morinaka, Y., Yamasaki, M., Kotake, T., Yamamoto, E., Sun, J., Hirano, K., Matsuoka, M. (2023) Integrated genome-wide differentiation and association analyses identify causal genes underlying breeding-selected grain quality traits in japonica rice. Mol. Plant. 16(9): 1460-1477.
• Ghosh K., Takahashi D., Kotake T. (2023) Plant type II arabinogalactan: Structural features and modification to increase functionality. Carbohydr. Res. 529: 108828.
• Oota, M., Toyoda, S., Kotake, T., Wada, N., Hashiguchi, M., Akashi, R., Tsai A.Y., Sawa, S. (2023) Rhamnogalacturonan-I as a nematode chemoattractant from Lotus corniculatus Super-growing Root culture. Front. Plant Sci., 13: 1008725.
• Kutsuno,T., Chowhan, S., Kotake, T., Takahashi D. (2023) Temporal cell wall changes during cold and deacclimation and its potential involvement in growth and freezing tolerance. Physiol. Plant., 175: e13837.
• Teh O.-K., Singh P., Ren J., Huang L.-T., Ariyarathne M., Salamon B.P., Wang Y., Kotake T., Fujita T. (2022) Surface-localised glycoproteins act through class C ARFs to fine-tune gametophore initiation in Physcomitrium patens. Development 149: dev200370.
• Kikuchi A., Hara K., Yoshimi Y., Soga K., Takahashi D., Kotake T. (2022) In vivo structural modification of type II arabinogalactans with fungal endo-β-1,6-galactanase in Arabidopsis. Front. Plant Sci., 13: 1010492.
• Miyagi A., Mori K., Ishikawa T., Ohkubo S., Adachi S., Yamaguchi M., Ookawa T., Kotake T., Kawai-Yamada M. (2022) Metabolomic analysis of rice brittle culm mutants revealed each mutant- specific metabolic pattern in each organ. Metabolomics., 18: 95.
• Md Sakil A., Mukae, K., Funada, R., Kotake, T., Ueno, S., Aktar M.M.1,5, Md. Shyduzzaman R., Inoue-Aono, Y., Moriyasu, Y.(2022) Amino acids supplied through the autophagic/endocytic pathway promote starch synthesis in Physcomitrella protonemal cells. Plants 11., 2157 . DOI: 10.3390/plants11162157.
• Feijao, C.V., Morreel, K., Anders, N., Tryfona, T., Busse-Wicher, M., Kotake, T., Boerjan, W., and Dupree, P. (2022) Hydroxycinnamic acid-modified xylan side chains and their cross-linking products in rice cell walls are reduced in the Xylosyl arabinosyl substitution of xylan 1 mutant. Plant J., 109: 1152-1167.
• Nishigaki, N., Yoshimi, Y., Kuki, H., Kunieda, T., Hara-Nishimura, I., Tsumuraya, Y., Takahashi, D., Dupree, P. and Kotake, T. (2021) Galactoglucomannan structure of Arabidopsis seed-coat mucilage in GDP-mannose synthesis impaired mutants. Physiol. Plant., 173: 1244-1252.
• Tsai, A. Y. L., Iwamoto, Y., Tsumuraya, Y., Oota, M., Konishi, T., Ito, S., Kotake, T., Ishikawa, H. and Sawa, S. (2021) Root-knot nematode chemotaxis is positively regulated by L-galactose sidechains of mucilage carbohydrate rhamnogalacturonan-I. Sci. Adv., 7: eabh4182.
• Cao, Y.L. et al. (2021) Wolfberry genomes and the evolution of Lycium (Solanaceae). Commun. Biol., 4: 671.
• Kondo, T., Kichijo, Y., Nakaya, M., Takenaka, S., Arakawa, T., Kotake, T., Fushinobu, S., and Sakamoto, T. (2021) Biochemical and structural characterization of a novel 4-O-α-L-rhamnosyl-β-D-glucuronidase from Fusarium oxysporum. FEBS J., 288: 4918-4938.
• Matsuyama, K., Kishine, N., Fujimoto, Z., Sunagawa, N., Kotake, T., Tsumuraya, Y., Samejima, M., Igarashi, K. and Kaneko, S. (2020) Crystal structure of GH43 exo-β-1,3-galactanase from the basidiomycete Phanerochaete chrysosporium provides insights into the mechanism of bypassing side chains. J. Biol. Chem., 295: 18539-18552.
• Lopez-Hernandez, F., Tryfona, T., Rizza, A., Yu, X., Harris, M.O., Webb, A.A.R., Kotake, T. and Dupree, P. (2020) Calcium binding by arabinogalactan polysaccharides is important for normal plant development. Plant Cell 32., 3346-3369.
• Yoshimi Y., Hara K., Yoshimura M., Tanaka N., Higaki T., Tsumuraya Y. and Kotake T. (2020) Expression of a fungal exo-β-1,3-galactanase in Arabidopsis reveals a role of type II arabinogalactan in the regulation of cell shape. J. Exp. Bot. 71., 5414-542.
• Tsumuraya, Y., Ozeki, E., Ooki, Y., Yoshimi, Y., Hashizume, K. and Kotake, T. (2019) Properties of arabinogalactan-proteins in European pear (Pyrus communis L.) fruits. Carbohydr. Res.,485: 107816.
• Sato, K., Hara, K., Yoshimi, Y., Kitazawa, K., Ito, H., Tsumuraya, Y. and Kotake, T. (2018) Yariv reactivity of type II arabinogalactan from larch wood. Carbohydr. Res., 467: 8-13.

エコトキシコロジーユニット

• Morishita M, Arai H, Ueda C, Arai Y, Ishii H, Tsukahara S. (2025) Sex difference in vasoactive intestinal polypeptide neural projection in the limbic system of the mouse brain. Endocrinology, 166, bqaf150, doi: 10.1210/endocr/bqaf150.
• Arai Y and Tsukahara S. (2025) A male-dominant cell group expressing calbindin-D28K and androgen receptor in the mouse preoptic area requires postnatal testicular androgens and histone deacetylation. J Neuroendocrinol, doi: 10.1111/jne.70097.
• Koiso R, Kushida C, Kanaya M, Tsukahara S. (2025) Emergence of the sex difference in calbindin D-28K neurons and the role of calbindin D-28K in clustering neurons of the preoptic area in mice. Cell Tissue Res., 401, 117–128, doi: 10.1007/s00441-025-03981-3.
• Suzuki M and Tsukahara S. (2025) In ovo exposure to ethynylestradiol demasculinizes the vasotocin system in the medial preoptic nucleus in male Japanese quail. Neurosci Lett., 864, 138310, doi: 10.1016/j.neulet.2025.138310.
• Abe N, Sakiyama A, Suzuki M , Win-Shwe TT, Suzuki T, Kawashima T, Tsukahara S. (2024) Ethynylestradiol feminizes gene expression partly in testis developing as ovotestis and disrupts asymmetric müllerian duct development by eliminating asymmetric gene expression in Japanese quail embryos. Toxicol Sci., 199:210-226.
• Win-Shwe TT., Abe N., Sakiyama A., Suzuki M., Sano K., Kawashima T., Tsukahara S. (2024) In ovo o,p'-DDT exposure induces malformation of reproductive organs and alters the expression of genes controlling sexual differentiation in Japanese quail embryo. J Appl Toxicol.,  44:699-711.
• Morishita M, Kobayashi K, Mitsuzuka M, Takagi R, Ono K, Momma R, Tsuneoka Y, Horio S, Tsukahara S. (2023) Two-Step Actions of Testicular Androgens in the Organization of a Male-Specific Neural Pathway from the Medial Preoptic Area to the Ventral Tegmental Area for Modulating Sexually Motivated Behavior. J Neurosci., 43:7322-7336.
• Tsukahara, S., Morishita, M., Sasaki, S., Wakayama, K., Kobayashi, K., Ohno, K., Kawashima T. (2021) Sexually dimorphic expression of sexual differentiation genes in the internal genital organs of Japanese quail embryos. General Comp Endocrinol., 314: 113917.
• Morishita, M., Kamada, A., Tsukahara, S. (2021) Neuronal activation of the sexually dimorphic nucleus of the preoptic area in female and male rats during copulation and its sex differences, Neurosci Lett., 755: 135915.
• Tsukahara, S., Morishita M. (2020) Sexually dimorphic formation of the preoptic area and the bed nucleus of the stria terminalis by neuroestrogens. Front Neurosci., 14: 797,
• Ogawa, S., Tsukahara, S., Choleris, E., Vasudevan, N. (2020) Estrogenic regulation of social behavior and sexually dimorphic brain formation. Neurosci Biobehav Rev., 110: 46-59.
• Okino, E., Morita, S., Hoshikawa, Y., Tsukahara, S. (2020) The glutamatergic system in the preoptic area is involved in the retention of maternal behavior in maternally experienced female rats. Psychoneuroendocrinology., 120: 104792.
• Morishita, M., Koiso, R., Tsukahara S. (2020) Actions of peripubertal gonadal steroids in the formation of sexually dimorphic brain regions in mice. Endocrinology., 161: 1-17.
• Sano, K., Matsukami, H., Suzuki, G., Htike, NTT., Morishita, M., Win-Shwe, TT., Hashimoto, S., Kawashima, T., Isobe, T., Nakayama, SF., Tsukahara, S., Maekawa F. (2020) Estrogenic action by tris(2,6-dimethylphenyl) phosphate impairs the development of female reproductive functions. Environ Int., 138: 105662.
• Nakashima, S., Morisita, M., Ueno, K., Tsukahara, S. (2019) Region-specific effects of copulation on dendritic spine morphology and gene expression related to spinogenesis in the medial preoptic nucleus of male rats. Psychoneuroendocrinology., 108: 1-13.
• Kanaya, M., Morishita, M., Tsukahara, S. (2018) Temporal expression patterns of genes related to sex steroid action in sexually dimorphic nuclei during puberty. Front Endocrinol., 9: 213.
• Maejima, S., Abe, Y., Yamaguchi, S., Musatov, S., Ogawa, S., Kondo, Y., Tsukahara, S. (2018) VGF in the medial preoptic nucleus increases sexual activity following sexual arousal induction in male rats. Endocrinology., 159: 3993-4005.

脂質科学ユニット

• Ishikawa, T. (2026) Advanced lipidomic techniques for high-throughput profiling of complex sphingolipids in plant tissues. J. Plant Res., in press.
• Wada, M., Kuga, C., Atsuzawa, K., Miyagi, A., Ishikawa, T., Yamaguchi, M., Fujiki, Y., Kaneko, Y., Kawai-Yamada, M., Nishida, I. (2026) Genetic defects in the CDP-choline pathway for phosphatidylcholine biosynthesis cannot be transmitted to offspring via male gametophytes owing to interruption of autophagy-like processes required for pollen germination in Arabidopsis thaliana. J. Plant Res., in press.
• Akashi, K., Kodama, Y., Sakaguchi, H., Hashida, S., Miyagi, A., Ishikawa, T., Yamaguchi, M., Kawai-Yamada, M. (2025) Identification of CCR4C as a chloroplast-localized NADP(H) phosphatase regulating NAD(P)(H) balance in Arabidopsis. Proc. Natl. Acad. Sci. USA, 122, e2504605122.
• Akamatsu, A., Ishikawa, T., Tanaka, H., Kawano, Y., Hayashi, M., Takeda, N. (2025) Accumulation of phosphatidylinositol 4,5-bisphosphate inhibits the excessive infection of rhizobia in Lotus japonicus. New Phytol., in press.
• Umemura, Y., Islam, M., Hasi, R.Y., Ishikawa, T., Nagano, M., Oka, T., Komura, N., Imamura, A., Ishida, H., Ando, H., Tanaka, T., Tanaka, H. (2025) Chemical Synthesis and Structural Determination of the Inositol Glycan Head of Plant Sphingolipid GIPC in Brassicaceae. Chem. Eur. J., 31, e01987.
• Islam, M., Hasi, R.Y., Umemura, Y., Tanaka, H., Kondo, Y., Ishikawa, T., Nagano, M., Ali, H., Kawakami, R., Aihara, M., Tanaka, T. (2025) Method for isolation and quantification of inositol glycan produced by glycosylinositol phosphoceramide-hydrolysing phospholipase D in plants. J. Biochem., 177, 387-394.
• Tanikawa, R., Sakaguchi, H., Ishikawa, T. Hihara, Y. (2025) Accumulation of acyl plastoquinol and triacylglycerol in six cyanobacterial species with different sets of genes encoding type-2 diacylglycerol acyltransferase-like proteins. Plant Cell Physiol., 66: 15-22.
• Kadeer, A., Ishikawa, Y., Dayarathne, K., Miyagi, A., Ishikawa, T., Yamaguchi, M., Kawai-Yamada, M. (2024) The deficiency of methylglyoxal synthase promotes cell proliferation in Synechocystis sp. PCC 6803 under mixotrophic conditions. Plant Biotech., 41: 393-399.
• Dayarathne, K., Ishikawa, T., Kadeer, A., Yamaguchi, M., Kawai-Yamada, M. (2024) The effect of light availability and light wavelength on growth, 2-MIB biosynthesis, and 2-MIB-related gene expression in Pseudanabaena foetida var. intermedia. Arch. Microb., 206: 367.
• Urano, K., Oshima, Y., Ishikawa, T., Kajino, T., Sakamoto, S., Sato, M., Toyooka, K., Fujita, M., Kawai-Yamada, M., Taji, T., Maruyama, K., Yamaguchi-Shinozaki, K., Shinozaki K. (2024) Arabidopsis DREB26/ERF12 and its close relatives regulate cuticular wax biosynthesis under drought stress condition. Plant J., 120: 2057-2075.
• Sun, P., Zhao, J., Sha, G., Zhou, Y., Zhao, M., Li, R., Kong, X., Sun, Q., Li, Y., Li, K., Bi, R., Yang, L., Qin, Z., Huang, W., Wang, Y., Gao, J., Chen, G., Zhang, H., Adnan, M., Yang, L., Zheng, L., Chen, X., Wang, G., Ishikawa, T., Li, Q., Xu, J., Li, G. (2024) Inhibitor of cardiolipin biosynthesis-related enzyme MoGep4 confers broad-spectrum anti-fungal activity. Plant Cell Environ., 47, 4259-4274.
• Kurima, K., Jimbo, H., Takashi, F., Saito, M., Ishikawa, T., Wada, H. (2024) High myristic acid in glycerolipids enhances the repair of photodamaged photosystem II under strong light. Plant Cell Physiol., 65, 790-797.
• Ishikawa, T., Domergue, D., Amato, A., Corellou, F. (2024) Characterization of unique eukaryotic sphingolipids with temperature-dependent Δ8-unsaturation from the picoalga Ostreococcus tauri. Plant Cell Physiol., 65, 1029-1046.
• Wang, M., Tabeta, H., Ohtaka, K., Kuwahara, A., Nishihama, R., Ishikawa, T., Toyooka, K., Sato, M., Wakazaki, M., Akashi, H., Tsugawa, H., Shoji, T., Okazaki, Y., Yoshida, K., Sato, R., Ferjani, A., Kohchi, T., Yokota-Hirai, M. (2024) The phosphorylated pathway of serine biosyntｓhesis affects sperm, embryo, and sporophyte development, and metabolism in Marchantia polymorpha. Commun. Biol., 7, 102.
• Ushio, M., Ishikawa, T., Matsuura, T., Mori, I.C., Kawai-Yamada, M., Fukao, Y., Nagano, M. (2023) MHP1 and MHL generate odd-chain fatty acids from 2-hydroxy fatty acids in sphingolipids and are related to immunity in Arabidopsis thaliana. Plant Sci., 336, 111840.
• Takai, Y., Hasi, R.Y., Matsumoto, N., Fujita, C., Ali, H., Hayashi, J., Kawakami, R., Aihara, M., Ishikawa, T., Imai, H., Wakida, M., Ando, K., Tanaka, T. (2023) Degradation of glycosylinositol phosphoceramide during plant tissue homogenization. J. Biochem., 175, 115-124.
• Dayarathne, K., Ishikawa, T., Watanabe, S., Ishikawa, Y., Aikeranmu, K., Kitagawa, H., Komatsubara, N., Yamaguchi, M., Kawai-Yamada, M. (2023) Heterologous expression of mtf and 1 mtc genes of Pseudanabaena foetida var. intermedia is sufficient to produce 2-methylisoborneol in Escherichia coli. Microbiol. Spectr., 11, e02561-23.
• Chaomurilege, Miyagi, A., Ishikawa, T., Yamaguchi, M., Murayama, H., Kawai-Yamada, M. (2023) Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants. Plant Signal. Behav., 18, 2215618.
• Yang, M., Sakuraba, Y., Ishikawa, T., Ohtsuki, N., Kawai-Yamada, M., Yanagisawa, S. (2023) Chloroplastic Sec14-like proteins modulate growth and phosphate deficiency responses in Arabidopsis and rice. Plant Physiol., 192, 3030-3048.
• Ishikawa, T., Takano, S., Tanikawa, R., Fujihara, T., Atsuzawa, K., Kaneko, Y., Hihara, Y. (2023) Acylated plastoquinone is a novel neutral lipid accumulated in cyanobacteria. PNAS Nexus, 2, pgad092
• Suzuki, S., Tanaka, D., Miyagi, A., Takahara, K., Kono, M., Chaomurilege, Noguchi K., Ishikawa, T., Nagano, M., Yamaguchi, M., Kawai-Yamada M. (2022) Loss of peroxisomal NAD kinase 3 (NADK3) affects photorespiration metabolism in Arabidopsis. J. Plant Physiol., 283, 153950.
• Hasi, RY., Ishikawa, T., Sunagawa, K., Takai, Y., Ali, H., Hayashi, J., Kawakami, R., Yuasa, K., Aihara, M., Kanemaru, K., Imai, H., Tanaka T. (2022) Nonspecific phospholipase C3 of radish has phospholipase D activity toward glycosylinositol phosphoceramide. FEBS Lett., 596, 3024-3036.
• Chaomurilege, Zu Y., Miyagi, A., Hashida, S., Ishikawa, T., Yamaguchi, M., Kawai-Yamada M (2022) Loss of chloroplast-localized NAD kinase causes ROS stress in Arabidopsis thaliana. J. Plant Res., 136, 97-106.
• Miyagi, A., Mori, K., Ishikawa, T., Ohkubo, S., Adachi, S., Yamaguchi, M., Ookawa, T., Kotake, T., Kawai‑Yamada M (2022). Metabolomic analysis of rice brittle culm mutants reveals each mutant‑ specific metabolic pattern in each organ. Metabolomics., 18, 95.
• Ailizati, A., Nagahage, ISP., Miyagi, A., Ishikawa, T., Kawai-Yamada M., Demura, T., Yamaguchi M (2022) VND-INTERACTING2 effectively inhibits transcriptional activities of VASCULAR-RELATED NAC-DOMAIN7 through a conserved sequence. Plant Biotechnol., 39, 147-153.
• Ukawa, T., Banno, F., Ishikawa, T., Kasahara, K., Nishina, Y., Inoue, R., Tsujii, K., Yamaguchi, M., Takahashi, T., Fukao, F., Kawai-Yamada M., Nagano, M. (2022) Sphingolipids with 2-hydroxy fatty acids aid in plasma membrane nanodomain organization and oxidative burst. Plant Physiol., 189, 839-857.
• Steshin, M. and Ishikawa, T. (2021). Liquid chromatography-tandem mass spectrometry with a new separation mode for rapid profiling of the Z/E isomers of plant glucosylceramides. J. Chromatogr. B., 1178: 122807.
• Moore, W.M., Chan, C., Ishikawa, T., Rennie, E.A., Wipf, H.M.L., Benites, V., Kawai-Yamada, M., Mortimer, J.C. and Scheller, H.V. (2021) Reprogramming sphingolipid glycosylation is required for endosymbiont persistence in Medicago truncatula. Curr. Biol., 31: 2374-2385.
• Jing, B., Ishikawa, T., Soltis, N., Inada, N., Liang, Y., Murawska, G., Andeberhan, F., Pidatala, R., Yu, X., Baidoo, E., Kawai-Yamada, M., Loque, D., Kliebenstein, D.J., Dupree, P. and Mortimer, J.C. (2021) The Arabidopsis thaliana nucleotide sugar transporter GONST2 is a functional homolog of GONST1. Plant Direct., e00309.
• Ishikawa, Y., Cassan, C., Kadeer, A., Yuasa, K., Sato, N., Sonoike, K., Kaneko, Y., Miyagi, A., Takahashi, H., Ishikawa, T., Yamaguci, M., Nishiyama, Y., Hihara, Y., Gibon, Y. and Kawai-Yamada, M. (2021) The NAD kinase Slr0400 functions as a growth repressor in Synechocystis sp. PCC 6803. Plant Cell Physiol., 62: 668-677.
• Nagata, K., Ishikawa, T., Kawai-Yamada, M., Takahashi, T. and Abe, M. (2021) Ceramides mediate positional signals in Arabidopsis thaliana protoderm differentiation. Development., 148: dev194969.
• Sato, M., Nagano, M., Jin, S., Miyagi, A., Yamaguchi, M., Kawai-Yamada, M. and Ishikawa, T. (2019) Plant-unique cis/trans isomerism of long-chain base unsaturation is selectively required for aluminum tolerance resulting from glucosylceramide-dependent plasma membrane fluidity. Plants., 9: 19.
• Hasi, R.M., Miyagi, M., Morito, K., Ishikawa, T., Kawai-Yamada, M., Imai, H., Fukuta, T., Kogure, K., Kanemaru, K., Hayashi, J., Kawakami, R. and Tanaka, T. (2019) Glycosylinositol phosphoceramide-specific phospholipase D activity catalyzes transphosphatidylation. J. Biolchem., 166: 441-448.
• Ishikawa, Y., Miyagi, A., Ishikawa, T., Nagano, M., Yamaguchi, M., Hihara, Y., Kaneko, Y. and Kawai-Yamada, M. (2019) One of the NAD kinases, sll1415, is required for the glucose metabolism of Synechocystis sp. PCC 6803. Plant J., 98: 654-666.
• Ishikawa, T., Fang, L., Rennie, E.A., Sechet, J., Yan, J., Jing, B., Moore, W., Cahoon, E.B., Scheller, H.V., Kawai-Yamada, M. and Mortimer, J.C. (2018) GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) is a GlcNAc-containing glycosylinositol phosphorylceramide glycosyltransferase. Plant Physiol., 177: 938-952.
• Sechet, J., Htwe, S., Urbanowicz, B., Agyeman, A., Feng, W., Ishikawa, T., Dinneny, J., Colomes, M., Satish Kumar, K., Kawai-Yamada, M., O'Neill, M. and Mortimer, J.C. (2018) Suppressing Arabidopsis GGLT1 affects growth by reducing the L-galactose content and borate cross-linking of rhamnogalacturonan II. Plant J., 96: 1036-1050.